ELISA/assay

Rat IL-6 PicoKine ELISA Kit

MBS175908

96 Wells

440 USD

ELISA Kit

Rat IL-6 PicoKine ELISA Kit

IL-6

Interleukin-6; interleukin 6; B cell differentiation factor ; B cell stimulatory factor 2; B-cell stimulatory factor 2; BSF 2; BSF-2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor ; Hepatocyte stimulating fact; HGF; HPGF; HSF ; Hybridoma growth factor; Hybridoma plasmacytoma growth factor ; IFN-beta-2; IFNB2 ; IL 6; IL-6; IL6; IL6 protein ; IL6_HUMAN; Interferon beta 2; Interferon beta-2; Interleukin 6; Interleukin 6 (interferon beta 2) ; Interleukin BSF 2; Interleukin-6; Interleukin6

Interleukin-6; Interleukin-6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); interleukin 6

IL6

Il6; Il6; ILg6; Ifnb2; Il-6; IL-6

IL6_RAT

Rat

211

Natural and recombinant rat IL-6

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate). Assay Type: Sandwich. Detection Range: 62.5pg/ml-4000pg/ml. Sensitivity: < 5pg/ml

Principle of the assay: rat IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli,F25-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-6 amount of sample captured in plate. Background: Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene.1 Interleukin-6 (IL-6) is involved not onlyin the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.2 IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption.3 IL-6 has beensuggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH).4 Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates acell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130.5 The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids withthe molecular mass of 21.8kDa.

124350

P20607.1

P20607

24,357 Da

Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198529); Cellular Senescence Pathway (1010386); Cellular Responses To Stress Pathway (1010374); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795)

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

96 Wells

440 USD