ELISA/assay

Rat IGF-2 PicoKine ELISA Kit

MBS175899

96 Wells

455 USD

ELISA Kit

Rat IGF-2 PicoKine ELISA Kit

IGF-2

Insulin-like growth factor II; insulin-like growth factor 2 (somatomedin A); C11orf43; FLJ22066; FLJ44734; IGF 2; IGF II; IGF-II; IGF2; IGF2_HUMAN; IGFII; INSIGF; Insulin like Growth Factor 2; Insulin like growth factor 2 (somatomedin A); Insulin like growth factor II; Insulin like growth factor II precursor; Insulin like growth factor type 2; pp9974; Preptin; Putative insulin like growth factor II associated protein; Somatomedin A; Somatomedin-A

Insulin-like growth factor II; Insulin-like growth factor II; insulin-like growth factor II; MSA; IGF-II; multiplication-stimulating activity; insulin-like growth factor II (IGF-II); multiplication-stimulating polypeptide; Insulin-like growth factor II (somatomedin A); insulin-like growth factor II isoform 2 variant 4 preprotein; insulin-like growth factor 2; Multiplication-stimulating activity; MSA; Multiplication-stimulating polypeptideCleaved into the following 2 chains:Insulin-like growth factor II; Preptin

IGF2

Igf2; Igf2; IGFII; RNIGF2; Igf-2; IGF-II; MSA

IGF2_RAT

Rat

180

Natural and recombinant rat IGF-2

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 62.5pg/ml-4000pg/ml. Sensitivity: < 5pg/ml

Principle of the assay: rat IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-2 has been precoated onto 96-well plates. Standards(E.coli,A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGF-2 amount of sample captured in plate. Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth.1 It is paternally expressed in the fetus and placenta.2IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues.3 IGF-II appears to be induced by placental lactogen during prenatal development.4 It is amediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.5

124258

P01346.1

P01346

20,086 Da

Apoptosis Pathway (219785); Endochondral Ossification Pathway (219771); Hedgehog Signaling Pathway (198499); Hemostasis Pathway (1010891); IGF1R Signaling Cascade Pathway (1010238); IRS-related Events Triggered By IGF1R Pathway (1010240); Metabolism Of Proteins Pathway (1009706); Platelet Activation, Signaling And Aggregation Pathway (1010901); Platelet Degranulation Pathway (1010913); Proteoglycans In Cancer Pathway (782034)

a mitogenic growth factor; may have a role in fetal development [RGD, Feb 2006]

96 Wells

455 USD