Human P-Cadherin PicoKine ELISA Kit | MyBioSource MBS175869 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human P-Cadherin PicoKine ELISA Kit

catalog :

MBS175869

quantity :

96 Wells

price :

455 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175869

products type :

ELISA Kit

products full name :

Human P-Cadherin PicoKine ELISA Kit

products short name :

P-Cadherin

products name syn :

Cadherin-3; cadherin 3, type 1, P-cadherin (placental); AI385538; CADH3_HUMAN; Cadherin 3; Cadherin 3 precursor; Cadherin 3 type 1; Cadherin-3; Cadp; Calcium dependent adhesion protein placental; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; P cadherin (placental); P-cadherin; PCAD; Placental cadherin

other names :

Cadherin-3; Cadherin-3; cadherin-3; calcium-dependent adhesion protein, placental; cadherin 3, type 1, P-cadherin (placental); Placental cadherin; P-cadherin

products gene name :

CDH3

other gene names :

CDH3; CDH3; CDHP; HJMD; PCAD; CDHP; P-cadherin

uniprot entry name :

CADH3_HUMAN

reactivity :

Human

sequence length :

829

specificity :

Natural and recombinant human P-Cadherin

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

other info1 :

Samples: Cell culture supernates and serum. Assay Type: Sandwich. Detection Range: 62.5pg/ml-4000pg/ml. Sensitivity: < 2pg/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

products description :

Principle of the assay: human P-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P-Cadherin has been precoated onto 96-well plates. Standards(NSO,D108 - G654) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P-Cadherin amount of sample captured in plate. Background: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

ncbi gi num :

146345382

ncbi acc num :

P22223.2

uniprot acc num :

P22223

ncbi mol weight :

86,552 Da

ncbi pathways :

Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532)

ncbi summary :

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq, Jul 2008]

uniprot summary :

CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: cell-cell adherens junction; cytoplasm; integral to membrane; plasma membrane. Molecular Function: calcium ion binding. Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; response to drug; intercellular junction assembly and maintenance; wound healing; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; negative regulation of transforming growth factor-beta2 production; retinal homeostasis; hair cycle process; cell-cell adhesion; positive regulation of monophenol monooxygenase activity; negative regulation of catagen; visual perception; keratinization; cell adhesion; homophilic cell adhesion. Disease: Ectodermal Dysplasia, Ectrodactyly, And Macular Dystrophy Syndrome; Hypotrichosis, Congenital, With Juvenile Macular Dystrophy

size1 :

96 Wells

price1 :

455 USD

more info or order :

MyBioSource product webpage