ELISA/assay

Human G-CSF PicoKine ELISA Kit

MBS175868

1 x96 Wells

305 USD

ELISA Kit

Human G-CSF PicoKine ELISA Kit

G-CSF

Granulocyte colony-stimulating factor; colony stimulating factor 3 (granulocyte); C17orf33; Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; MGI IG; OTTMUSP00000006785; Pluripoietin; RP23-395E10.4

Granulocyte colony-stimulating factor; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte); Pluripoietin; INN: Filgrastim; INN: Lenograstim

CSF3

CSF3; CSF3; GCSF; CSF3OS; C17orf33; C17orf33; GCSF; G-CSF

CSF3_HUMAN

Human

207

Natural and recombinant human G-CSF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: For quantitative detection of human G-CSF in cell culture supernates, serum and plasma(heparin, EDTA, citrate). Detection Range: 31.2pg/ml-2000pg/ml

Sensitivity: < 4pg/ml

Principle of the assay: human G-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for G-CSF has been precoated onto 96-well plates. Standards(E.coli,T31-P204) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for G-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human G-CSF amount of sample captured in plate. Background: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture.1 G-CSF is also termed colonystimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity.2 Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Statpathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant humanG-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

117564

P09919.1

P09919

18,434 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response Pathway 198794!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!Malaria Pathway 152665!!Malaria Pathway 152657!!PI3K-Akt Signaling Pathway 692234

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

1 x96 Wells

305 USD