ELISA/assay

Rat NGF/NGF beta PicoKine ELISA Kit

MBS175864

96 Wells

360 USD

ELISA Kit

Rat NGF/NGF beta PicoKine ELISA Kit

NGF/NGF beta

Beta-nerve growth factor; nerve growth factor (beta polypeptide); Beta nerve growth factor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide); Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGFB; NID67

Beta-nerve growth factor; Beta-nerve growth factor; beta-nerve growth factor; nerve growth factor (beta polypeptide)

NGF

Ngf; Ngf; Ngfb; beta-NGF; Ngfb; Beta-NGF

NGF_RAT

Rat

241

Natural and recombinant rat NGF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates and serum. Assay Type: Sandwich. Detection Range: 15.6pg/ml-1000pg/ml. Sensitivity: < 1pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Background/Introduction: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2. Principle of the Assay: The rat NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat NGF amount of sample captured in plate.

1352495

P25427.2

P25427

27,010 Da

ARMS-mediated Activation Pathway (1010106); Activation Of TRKA Receptors Pathway (1010097); Apoptosis Pathway (83452); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (1010093); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1010083); Ceramide Signalling Pathway (1010091); Frs2-mediated Activation Pathway (1010105); Id Signaling Pathway (198536); Inflammatory Mediator Regulation Of TRP Channels Pathway (948290)

96 Wells

360 USD