ELISA/assay

Human TrkA PicoKine ELISA Kit

MBS175860

96 Wells

475 USD

ELISA Kit

Human TrkA PicoKine ELISA Kit

TrkA

High affinity nerve growth factor receptor; neurotrophic tyrosine kinase, receptor, type 1; High affinity nerve growth factor receptor; MTC; Neurotrophic tyrosine kinase receptor type 1; NTRK1; NTRK1_HUMAN; Oncogene TRK; p140-TrkA; TRK; Trk-A; TRK1; TRK1-transforming tyrosine kinase protein

High affinity nerve growth factor receptor; High affinity nerve growth factor receptor; high affinity nerve growth factor receptor; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein; neurotrophic tyrosine kinase, receptor, type 1; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; Trk-A; gp140trk; p140-TrkA

NTRK1

NTRK1; NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; MTC; TRK; TRKA; Trk-A

NTRK1_HUMAN

Human

796

Natural and recombinant human TrkA

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, Cell lysates and tissue homogenates. Assay Type: Sandwich. Detection Range: 156pg/ml-10,000pg/ml. Sensitivity: < 10pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the assay: human TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TrkA amount of sample captured in plate. Background: Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able toactivate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are otherneurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found inkehumanoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4The standard product used in this kit is extracellular part (A33-P418) of recombinant human TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.

94730402

P04629.4

P04629

77,145 Da

ARMS-mediated Activation Pathway (106466); Activation Of TRKA Receptors Pathway (106456); Apoptosis Pathway (83060); Apoptosis Pathway (470); BDNF Signaling Pathway (712093); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Frs2-mediated Activation Pathway (106465); Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307)

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described. Protein type: EC 2.7.10.1; Membrane protein, integral; Oncoprotein; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; TK group; Trk family. Chromosomal Location of Human Ortholog: 1q21-q22. Cellular Component: protein complex; cell surface; integral to plasma membrane; late endosome membrane; early endosome; dendrite; cell soma; axon; early endosome membrane; late endosome; plasma membrane; cytoplasmic vesicle; receptor complex; endosome. Molecular Function: neurotrophin p75 receptor binding; neurotrophin binding; protein binding; protein homodimerization activity; nerve growth factor receptor activity; ephrin receptor binding; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; positive regulation of synaptic transmission, glutamatergic; olfactory nerve development; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; sympathetic nervous system development; response to radiation; learning and/or memory; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to axon injury; detection of temperature stimulus involved in sensory perception of pain; response to electrical stimulus; response to hydrostatic pressure; aging; response to drug; response to nutrient levels; phosphoinositide-mediated signaling; adenylate cyclase activation; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of angiogenesis; positive regulation of programmed cell death; response to ethanol; phospholipase C activation; B cell differentiation; Ras protein signal transduction; positive regulation of Ras protein signal transduction; response to activity; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Thyroid Carcinoma, Familial Medullary; Insensitivity To Pain, Congenital, With Anhidrosis

96 Wells

475 USD