ELISA/assay

Mouse ICAM-1 PicoKine ELISA Kit

MBS175838

96 Wells

455 USD

ELISA Kit

Mouse ICAM-1 PicoKine ELISA Kit

ICAM-1

Intercellular adhesion molecule 1; intercellular adhesion molecule 1; BB2; CD 54; CD54; CD54 antigen; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM 1; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MGC6195; MyD10; P3.58; Surface antigen of activated B cells

Intercellular adhesion molecule 1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1; myD10; intercellular adhesion molecule 1; MALA-2; MyD10; CD_antigen: CD54

ICAM1

Icam1; Icam1; CD54; Ly-47; Icam-1; MALA-2; Icam-1; ICAM-1

ICAM1_MOUSE

Mouse

537

Natural and recombinant mouse sICAM-1

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum, plasma (heparin, FDrA) and tissue homogenates. Assay Type: Sandwich. Detection Range: 156pg/ml-10000pg/ml. Sensitivity: < 10pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The mouse ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ICAM-1 has been precoated onto 96-weli plates. Standards (Expression system for standard: NSO, Immunogen sequence: Q28-N485) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ICAM-1 amount of sample captured in plate. Background/Introduction: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1)2 iCAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class " antigens and is likely to play a role in inflammatory responses. 1 The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.

124099

P13597.1

P13597

58,753 Da

Adaptive Immune System Pathway (971210); African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (970814); Epstein-Barr Virus Infection Pathway (585576); Epstein-Barr Virus Infection Pathway (587115); Extracellular Matrix Organization Pathway (1001015); HTLV-I Infection Pathway (373903)

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta. Protein type: Immunoglobulin superfamily; Cell adhesion; Membrane protein, integral. Cellular Component: extracellular space; cell surface; focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; immunological synapse; lipid raft; external side of plasma membrane. Molecular Function: integrin binding; protein complex binding. Biological Process: regulation of cell adhesion; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; leukocyte adhesion; cell-cell adhesion; sensory perception of sound; positive regulation of actin filament polymerization; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; cell adhesion; acute inflammatory response to antigenic stimulus

96 Wells

455 USD