Mouse NGF/NGF beta PicoKine ELISA Kit | MyBioSource MBS175827 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse NGF/NGF beta PicoKine ELISA Kit

catalog :

MBS175827

quantity :

96 Wells

price :

360 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175827

products type :

ELISA Kit

products full name :

Mouse NGF/NGF beta PicoKine ELISA Kit

products short name :

NGF/NGF beta

products name syn :

Beta-nerve growth factor; nerve growth factor (beta polypeptide); Beta nerve growth factor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide); Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGFB; NID67

other names :

Beta-nerve growth factor; Beta-nerve growth factor; beta-nerve growth factor; beta-NGF; nerve growth factor, beta; nerve growth factor

products gene name :

NGF

other gene names :

Ngf; Ngf; Ngfb; Ngfb; Beta-NGF

uniprot entry name :

NGF_MOUSE

reactivity :

Mouse

sequence length :

241

specificity :

Natura! and recombinant mouse NGF

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

other info1 :

Samples: Cell culture supernates and serum. Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 1 pg/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

products description :

Principle of the Assay: The mouse NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for NGF has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGF amount of sample captured in plate.

ncbi gi num :

128162

ncbi acc num :

P01139.2

uniprot acc num :

P01139

ncbi mol weight :

27,077 Da

ncbi pathways :

ARMS-mediated Activation Pathway (970820); Activation Of TRKA Receptors Pathway (1000905); Apoptosis Pathway (83257); Apoptosis Pathway (470); Apoptosis Signaling Pathway (522973); Axonal Growth Stimulation Pathway (1000902); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1000892); Ceramide Signalling Pathway (1000900); Frs2-mediated Activation Pathway (971392); Id Signaling Pathway (198411)

uniprot summary :

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cytoplasmic membrane-bound vesicle; extracellular region. Molecular Function: metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity; receptor binding. Biological Process: positive regulation of neuron maturation; regulation of neuron differentiation; adult locomotory behavior; positive regulation of axon extension; regulation of neurotransmitter secretion; sensory perception of pain; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; memory; regulation of release of sequestered calcium ion into cytosol; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; positive regulation of cell proliferation; positive regulation of transcription factor activity; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; positive regulation of collateral sprouting; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; neurite development

size1 :

96 Wells

price1 :

360 USD

more info or order :

MyBioSource product webpage