Mouse B7-1/CD80 PicoKine ELISA Kit | MyBioSource MBS175825 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse B7-1/CD80 PicoKine ELISA Kit

catalog :

MBS175825

quantity :

96 Wells

price :

455 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175825

products type :

ELISA Kit

products full name :

Mouse B7-1/CD80 PicoKine ELISA Kit

products short name :

B7-1/CD80

products name syn :

T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7-1 antigen; B7; BB1; CD80; CD80_HUMAN; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

other names :

T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen; CD80 antigen; Activation B7-1 antigen; B7; CD_antigen: CD80

products gene name :

CD80

other gene names :

Cd80; Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17; B7; B7

uniprot entry name :

CD80_MOUSE

reactivity :

Mouse

sequence length :

306

specificity :

Natural and recombinant mouse CD80

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

other info1 :

Samples: Cell culture supernates and serum. Assay Type: Sandwich. Detection Range: 62.5pg/ml-4000pg/ml. Sensitivity: < 10pg/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

products description :

Principle of the assay: mouse CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD80 has been precoated onto 96-well plates. Standards(NSO, D37-K245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate. Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37—K245,which is composed of two single chains acids with the dipolymer.

ncbi gi num :

461607

ncbi acc num :

Q00609.1

uniprot acc num :

Q00609

ncbi mol weight :

24,051 Da

ncbi pathways :

Adaptive Immune System Pathway (971210); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (1001188); CD28 Dependent PI3K/Akt Signaling Pathway (1001189); CD28 Dependent Vav1 Pathway (1001190); CTLA4 Inhibitory Signaling Pathway (1001191); Cell Adhesion Molecules (CAMs) Pathway (83266)

uniprot summary :

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding. Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation

size1 :

96 Wells

price1 :

455 USD

more info or order :

MyBioSource product webpage