ELISA/assay

Mouse IGFBP-1 PicoKine ELISA Kit

MBS175824

96 Wells

440 USD

ELISA Kit

Mouse IGFBP-1 PicoKine ELISA Kit

IGFBP-1

Insulin-like growth factor-binding protein 1; insulin-like growth factor binding protein 1; AFBP; hIGFBP-1; IBP1; IGF-binding protein 1; IGF-BP25; Insulin-like growth factor-binding protein 1; Placental protein 12; PP12

Insulin-like growth factor-binding protein 1; Insulin-like growth factor-binding protein 1; insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1); insulin-like growth factor binding protein 1

IGFBP1

Igfbp1; Igfbp1; Igfbp-1; IBP-1; IGF-binding protein 1; IGFBP-1

IBP1_MOUSE

Mouse

272

Natural and recombinant mouse IGFBP-1

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 3pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The mouse IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A26-N272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP-1 amount of sample captured in plate. Background/Introduction: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth 2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and 11.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copy gene, located on chromosome 7s The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KOa.

408360142

P47876.2

P47876

29,570 Da

ATF4 Activates Genes Pathway (1000429); Metabolism Of Proteins Pathway (970583); Myometrial Relaxation And Contraction Pathways (198333); PERK Regulates Gene Expression Pathway (1000428); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (1000422); Unfolded Protein Response (UPR) Pathway (1000423)

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; growth factor binding; insulin-like growth factor II binding. Biological Process: regulation of insulin-like growth factor receptor signaling pathway; regulation of cell growth

96 Wells

440 USD