ELISA/assay

Human TIMP-1 PicoKine ELISA Kit

MBS175822

96 Wells

410 USD

ELISA Kit

Human TIMP-1 PicoKine ELISA Kit

TIMP-1

Metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5; CCR5_HUMAN; CD195; CHEMR13; CLGI; Collagenase inhibitor; EPA; EPO; Erythroid potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; HIV-1 fusion coreceptor; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP; TIMP 1; TIMP metallopeptidase inhibitor 1; TIMP1 protein; Tissue Inhibitor of metalloproteinase 1; Tissue inhibitor of metalloproteinases; Tissue inhibitor of metalloproteinases 1; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase; inhibitor

Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1

TIMP1

TIMP1; TIMP1; EPA; EPO; HCI; CLGI; TIMP; CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1

TIMP1_HUMAN

Human

207

Natural and recombinant human TIMP-1

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and saliva. Assay Type: Sandwich. Detection Range: 156pg/ml-10,000pg/ml. Sensitivity: < 5pg/ml

Principle of the assay: human TIMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-1 has been precoated onto 96-well plates. Standards (NSO,C24-A207) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-1 amount of sample captured in plate. Background: The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates humanand murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactivecomplexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard productused in this kit is natural TIMP-1 with the molecular mass of 22KDa.

135850

P01033.1

P01033

23,171 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Matrix Metalloproteinases Pathway (198900); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xp11.3-p11.23. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity. Biological Process: response to peptide hormone stimulus; platelet activation; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis

96 Wells

410 USD