ELISA/assay

Rat IL-18 PicoKine ELISA Kit

MBS175814

96 Wells

455 USD

ELISA Kit

Rat IL-18 PicoKine ELISA Kit

IL-18

Interleukin-18; interleukin 18; Iboctadekin; IFN gamma inducing factor; IFN-gamma-inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL-1 gamma; IL-18; IL1 gamma; IL18; IL18 protein; IL18_HUMAN; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interferon gamma-inducing factor; Interleukin 1 gamma; Interleukin 18; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320

Interleukin-18; Interleukin-18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; interleukin 18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma

IL18

Il18; Il18; IL-18; Igif; IL-18; IFN-gamma-inducing factor; IL-1 gamma

IL18_RAT

Rat

194

Natural and recombinant rat IL-18

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 15.6pg/ml-1000pg/ml. Sensitivity: < 1pg/ml

Principle of the assay: Rat IL-18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been precoated onto 96-well plates. Standards(E.coli, H37-S194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-18 amount of sample captured in plate. Background: Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

3219808

P97636.1

P97636

20,056 Da

African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Cytokine Signaling In Immune System Pathway (1011036); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125115); Cytosolic DNA-sensing Pathway (124833); Hypertrophy Model Pathway (198527); Immune System Pathway (1010924); Inflammatory Bowel Disease (IBD) Pathway (842782)

a pro-inflammatory cytokine; acts as a modulator of immune functions [RGD, Feb 2006]

96 Wells

455 USD