Human PLGF PicoKine ELISA Kit | MyBioSource MBS175808 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PLGF PicoKine ELISA Kit

catalog :

MBS175808

quantity :

96 Wells

price :

440 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175808

products type :

ELISA Kit

products full name :

Human PLGF PicoKine ELISA Kit

products short name :

PLGF

products name syn :

Placenta growth factor; placental growth factor; AI854365; D12S1900; MGC93298; Pgf; PGFL; PIGF; Placenta growth factor; Placental growth factor; Placental growth factor vascular endothelial growth factor related protein; PlGF; PlGF 2; PLGF_HUMAN; PlGF2; SHGC 10760; Vascular endothelial growth factor related protein

other names :

Placenta growth factor; Placenta growth factor; placenta growth factor; placental growth factor, vascular endothelial growth factor-related protein; placental growth factor

products gene name :

PGF

other gene names :

PGF; PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760; PGFL; PLGF; PlGF

uniprot entry name :

PLGF_HUMAN

reactivity :

Human

sequence length :

221

specificity :

Natural and recombinant human PLGF

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

other info1 :

Samples: Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine. Assay Type: Sandwich. Detection Range: 15.6pg/ml-1000pg/ml. Sensitivity: < 1pg/ml

products description :

Principle of the assay: human PLGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLGF has been precoated onto 96-well plates. Standards (E.coli,A21-R149) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLGF amount of sample captured in plate. Background: The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1,PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis andcollateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.

ncbi gi num :

17380553

ncbi acc num :

P49763.2

uniprot acc num :

P49763

ncbi mol weight :

19,338 Da

ncbi pathways :

Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085); Signal Transduction Pathway (477114)

ncbi summary :

This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

uniprot summary :

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q24.3. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity. Biological Process: response to drug; organ regeneration; female pregnancy; signal transduction; cellular response to hormone stimulus; positive regulation of angiogenesis; cell-cell signaling; ureteric bud branching; positive regulation of cell division; positive regulation of cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell differentiation

size1 :

96 Wells

price1 :

440 USD

more info or order :

MyBioSource product webpage