ELISA/assay

Mouse BDNF PicoKine ELISA Kit

MBS175800

96 Wells

325 USD

ELISA Kit

Mouse BDNF PicoKine ELISA Kit

BDNF

Brain-derived neurotrophic factor; brain-derived neurotrophic factor; Abrineurin; ANON2; Brain derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Brain-derived neurotrophic factor; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; anorexia BDNF; brain derived neurotrophic factor

BDNF

Bdnf; Bdnf; BDNF

BDNF_MOUSE

Mouse

249

Natural and recombinant mouse BDNF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma(hepark" EDTA, citrate). Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 2pg/rnl

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The mouse BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BDNF has been precoated onto 90-well plates. Standards(Expression system for standard: sf21 , Immunogen sequence: H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BDNF amount of sample captured in plate. Background/Introduction: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state4 It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury5

114901

P21237.1

P21237

28,123 Da

Alcoholism Pathway (585577); Alcoholism Pathway (587116); Cocaine Addiction Pathway (546267); Cocaine Addiction Pathway (546273); Huntington's Disease Pathway (83297); Huntington's Disease Pathway (512); MAPK Signaling Pathway (198294); MAPK Signaling Pathway (83245); MAPK Signaling Pathway (456); Neurotrophin Signaling Pathway (101156)

The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Sep 2015]

BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted; Cytokine; Cell development/differentiation; Secreted, signal peptide. Cellular Component: extracellular space; cell; cytoplasmic membrane-bound vesicle; dendrite; extracellular region; terminal button; perikaryon; synaptic vesicle; mitochondrial crista; cell soma; perinuclear region of cytoplasm; axon; cytoplasm. Molecular Function: protein binding; growth factor activity; neurotrophin TRKB receptor binding; receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; axon guidance; mechanoreceptor differentiation; behavioral fear response; mitochondrial electron transport, NADH to ubiquinone; axon extension; regulation of synaptic plasticity; positive regulation of long-term neuronal synaptic plasticity; regulation of inhibitory postsynaptic membrane potential; learning and/or memory; negative regulation of neuroblast proliferation; ureteric bud development; regulation of neuron apoptosis; dendrite development; regulation of retinal cell programmed cell death; feeding behavior; regulation of metabolic process; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; inner ear development; response to drug; nervous system development; neuron recognition; regulation of axon extension; regulation of short-term neuronal synaptic plasticity; learning; negative regulation of striated muscle development; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; axon target recognition; glutamate secretion; positive regulation of transcription factor activity; nerve development; regulation of excitatory postsynaptic membrane potential; positive regulation of neuron differentiation; gamma-aminobutyric acid signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

96 Wells

325 USD