ELISA/assay

Human TSP2 PicoKine ELISA Kit

MBS175793

96 Wells

380 USD

ELISA Kit

Human TSP2 PicoKine ELISA Kit

TSP2

Thrombospondin-2; thrombospondin 2; THBS2; Thrombospondin-2; TSP2_HUMAN

Thrombospondin-2; Thrombospondin-2; thrombospondin-2; thrombospondin 2

THBS2

THBS2; THBS2; TSP2; TSP2

TSP2_HUMAN

Human

1172

Natural and recombinant human TSP2

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and human milk. Assay Type: Sandwich. Detection Range: 156pg/ml-10,000pg/ml. Sensitivity: < 10pg/ml

Principle of the assay: human TSP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TSP2 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TSP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TSP2 amount of sample captured in plate. Background: Thrombospondin-2(TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.

215273908

P35442.2

P35442

129,991 Da

ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Malaria Pathway (152665); Malaria Pathway (152657); Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459)

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

THBS2: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disk disease (IDD); also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. Belongs to the thrombospondin family. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 6q27. Cellular Component: extracellular region; basement membrane. Molecular Function: heparin binding; protein binding; calcium ion binding. Biological Process: positive regulation of synaptogenesis; negative regulation of angiogenesis; cell adhesion. Disease: Intervertebral Disc Disease

96 Wells

380 USD