ELISA/assay

Human Eotaxin PicoKine ELISA Kit

MBS175786

1 x96 Wells

380 USD

ELISA Kit

Human Eotaxin PicoKine ELISA Kit

Eotaxin

Eotaxin; chemokine (C-C motif) ligand 11; C-C motif chemokine 11; CCL 11; CCL11; CCL11_HUMAN; Chemokine (C-C motif) ligand 11; Chemokine C C motif ligand 11; Chemokine CC Motif Ligand 11; Chemokine ligand 11; Eosinophil Chemotactic P; Eosinophil chemotactic protein; Eotaxin; Eotaxin 1; MGC22554; SCYA 11; SCYA11; Small inducible cytokine A11; Small inducible cytokine subfamily A (Cys Cys) member 11; Small inducible cytokine subfamily A member 11; Small-inducible cytokine A11

Eotaxin; Eotaxin; eotaxin; eotaxin-1; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); chemokine (C-C motif) ligand 11; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11

CCL11

CCL11; CCL11; SCYA11; SCYA11

CCL11_HUMAN

Human

97

Natural and recombinant human Eotaxin

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: For quantitative detection of human Eotaxin in cell culture supernates, serum and plasma(citrate,EDTA). Detection Range: 15.6pg/ml-1000pg/ml

Sensitivity: < 2pg/ml

Principle of the assay: human Eotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Eotaxin has been precoated onto 96-well plates. Standards(E.coli,G24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Eotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Eotaxin amount of sample captured in plate. Background: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes.1 Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis,allergic rhinitis, asthma and parasitic infections.2 The gene maps to chromosome 17 and is expressedconstitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes.3 The human eotaxin gene is cloned and found tobe 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.4.

1706661

P51671.1

P51671

10,732 Da

Asthma Pathway 83120!!Asthma Pathway 532!!CXCR3-mediated Signaling Events Pathway 138011!!Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!GPCR Ligand Binding Pathway 161020

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Sep 2014]

1 x96 Wells

380 USD