ELISA/assay

Mouse Cathepsin B PicoKine ELISA Kit

MBS175784

1 x96 Wells

455 USD

ELISA Kit

Mouse Cathepsin B PicoKine ELISA Kit

Cathepsin B

Cathepsin B; cathepsin B; aAmyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CPSB; CTSB; Cysteine protease

Cathepsin B; Cathepsin B; cathepsin B; cathepsin B1; cathepsin B; Cathepsin B1Cleaved into the following 2 chains:Cathepsin B light chain; Cathepsin B heavy chain

CTSB

Ctsb; Ctsb; CB

CATB_MOUSE

Mouse

339

Natural and recombinant mouse Cathepsin B

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: For quantitative detection of mouse Cathepsin B in cell culture supernates, serum and plasma(heparin, EDTA). Detection Range: 156pg/ml-10,000pg/ml

Sensitivity: < 5pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the assay: mouse Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin B has been precoated onto 96-well plates. Standards(NSO,H18-F339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cathepsin B amount of sample captured in plate. Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomalcysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression ofcathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B H18—F339, with the molecular mass of 43KDa.

115712

P10605.2

P10605

37,280 Da

Antigen Processing And Presentation Pathway 83271!!Antigen Processing And Presentation Pathway 485!!Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway 1001018!!Collagen Degradation Pathway 1001030!!Collagen Formation Pathway 1001016!!Degradation Of The Extracellular Matrix Pathway 1001028!!Extracellular Matrix Organization Pathway 1001015!!Immune System Pathway 971216!!Innate Immune System Pathway 971212!!Lysosome Pathway 99272

1 x96 Wells

455 USD