ELISA/assay

Human Activin A PicoKine ELISA Kit

MBS175781

96-Strip-Wells

380 USD

ELISA Kit

Human Activin A PicoKine ELISA Kit

[Activin A]

[Inhibin beta A chain; inhibin, beta A; Activin beta A chain; erythroid differentiation factor; erythroid differentiation protein; follicle stimulating hormone releasing protein; FSH releasing protein; Inhibin; inhibin beta A chain]

[Inhibin, beta A (Activin A, activin AB alpha polypeptide); inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide); inhibin, beta A; Inhibin, beta A (Activin A, activin AB alpha polypeptide)]

[INHBA]

[INHBA; INHBA; EDF; FRP]

A4D1W7_HUMAN

Human

426

Natural and recombinant human Activin A

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and saliva. Assay Type: Quantitative Sandwich. Detection Range: 15.6pg/ml- 1000pg/ml. Sensitivity: <12pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The Human Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(Expression system for standard: CHO ; Immunogen sequence: G311-S426) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. Acvidin-Biotin-Peroxidase Compiex was added and unbound conugates were washed away with PBS or TBS buffer HRP substrate TMB was used to visualize HRP enzymatic tion. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Activin A amount of sample captured in plate. Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of Ire transforming growth factor-beta superfamily, is expressed loca!ly by the mesenchymal component of the hemopoietlc microenvironment. its expression is regulated on the mRNA level by different cytoKines. and the biologilt activity of the protein is. controlled by several inhibitory molecules.' Inhibins and activins are members of ine transforming growth factor beta superfamily and are known to modulate the growth and differentiation of severe I cell types 2 Inhibins and activins inhibit and activate. respectively. the secretion of follitropin by the pituitary glanj. Inhibins/activins are Involved in regulating a number of diverse functions such as hypothalamic and pituitary horlnone secretion, gonadal hormone secretion, germ celi development and maturation. erythroid differentiation. insulin secretion. nerve cell survival embryonic axial development or bone growth depending on their subunit composition. Inhibins appear to oppose the functions of activins.

A4D1W7

47,442 Da

ALK1 Signaling Events Pathway (137968); Antagonism Of Activin By Follistatin Pathway (730350); Cardiac Progenitor Differentiation Pathway (712094); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Glycoprotein Hormones Pathway (160989); Integrated Pancreatic Cancer Pathway (711360); Metabolism Of Proteins Pathway (106230); Peptide Hormone Biosynthesis Pathway (160988); Peptide Hormone Metabolism Pathway (771603)

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008]

INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p15-p13. Cellular Component: extracellular region. Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; defense response; palate development; negative regulation of cell cycle; negative regulation of interferon-gamma biosynthetic process; odontogenesis; negative regulation of cell proliferation; cell surface receptor linked signal transduction; hair follicle development; cell-cell signaling; ovarian follicle development; erythrocyte differentiation; cell differentiation; cell cycle arrest; negative regulation of B cell differentiation; hemoglobin biosynthetic process; response to drug; nervous system development; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; negative regulation of follicle-stimulating hormone secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of cell growth; positive regulation of follicle-stimulating hormone secretion; cell development; growth; G1/S transition of mitotic cell cycle

96-Strip-Wells

380 USD