antibody

Anti-CISH antibody

MBS175768

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Antibody

Anti-CISH antibody

CISH

Cytokine-inducible SH2-containing protein(CIS); cytokine inducible SH2-containing protein; BACTS2 antibody; cis 1 antibody; CIS antibody; CIS-1 antibody; CIS1 antibody; CISH antibody; CISH_HUMAN antibody; Cytokine inducible inhibitor of signaling type 1B antibody; Cytokine inducible SH2 containing protein antibody; Cytokine-inducible SH2-containing protein antibody; G18 antibody; Protein G18 antibody; SOCS antibody; Suppressor of cytokine signaling antibody

Cytokine-inducible SH2-containing protein; Cytokine-inducible SH2-containing protein; cytokine-inducible SH2-containing protein; suppressor of cytokine signaling; cytokine-inducible inhibitor of signaling type 1B; cytokine inducible SH2-containing protein; CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS

CISH

CISH; CISH; CIS; G18; SOCS; CIS-1; BACTS2; G18; CIS; SOCS

CISH_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

258

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CISH (241-258aa LPLPRRMADYLRQYPFQL), identical to the related rat and mouse sequences.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Cytokine-inducible SH2-containing protein(CISH) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat. Background: CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide(LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.

13124022

Q9NSE2.1

Q9NSE2

30,734 Da

Cytokine Signaling In Immune System Pathway (366171); ECS Complex Pathway (413455); ECS Complex Pathway (468394); EPO Receptor Signaling Pathway (198882); Growth Hormone Receptor Signaling Pathway (477128); IL-2 Signaling Pathway (198885); IL-3 Signaling Pathway (198881); IL2-mediated Signaling Events Pathway (137976); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077)

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

CISH: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate- recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR). By a subset of cytokines including EPO/erythropoietin. Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. 3 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: cytoplasm; plasma membrane; cytosol. Molecular Function: protein binding; protein kinase inhibitor activity. Biological Process: cytokine and chemokine mediated signaling pathway; protein kinase C activation; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; regulation of cell growth; negative regulation of JAK-STAT cascade. Disease: Mycobacterium Tuberculosis, Susceptibility To; Bacteremia, Susceptibility To, 2; Malaria, Susceptibility To

0.1 mg

245 USD