Anti-Vinculin antibody | MyBioSource MBS175711 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Vinculin antibody

catalog :

MBS175711

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175711

products type :

Antibody

products full name :

Anti-Vinculin antibody

products short name :

Vinculin

products name syn :

Vinculin; vinculin; CMD1W antibody; CMH15 antibody; Metavinculin antibody; MVCL antibody; OTTHUMP00000019861 antibody; OTTHUMP00000019862 antibody; VCL antibody; VINC antibody; VINC_HUMAN antibody; Vinculin antibody

other names :

Vinculin; Vinculin; vinculin; metavinculin; epididymis luminal protein 114; vinculin; Metavinculin; MV

products gene name :

VCL

other gene names :

VCL; VCL; MV; MVCL; CMD1W; CMH15; HEL114; MV

uniprot entry name :

VINC_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

sequence length :

1134

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin (173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a 3-generation family, Mulligan et al.(1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is though to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater-than-95% sequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids.

ncbi gi num :

21903479

ncbi acc num :

P18206.4

uniprot acc num :

P18206

ncbi mol weight :

24,904 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

ncbi summary :

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Cell adhesion. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: focal adhesion; costamere; protein complex; extracellular region; intercellular junction; fascia adherens; cytosol; actin cytoskeleton; cell-matrix junction; cell-cell adherens junction; adherens junction; cytoskeleton; plasma membrane; vesicle. Molecular Function: protein binding; cadherin binding; beta-catenin binding; structural molecule activity; actin binding; alpha-catenin binding. Biological Process: lamellipodium biogenesis; platelet activation; platelet degranulation; apical junction assembly; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell adhesion; cell motility; blood coagulation; negative regulation of cell migration. Disease: Cardiomyopathy, Dilated, 1w

size1 :

0.1 mg

price1 :

245 USD

more info or order :

MyBioSource product webpage