antibody

Anti-CCR5 antibody

MBS175709

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-CCR5 antibody

CCR5

C-C chemokine receptor type 5(C-C CKR-5/CC-CKR-5/CCR-5/CCR5); chemokine(C-C motif) receptor 5(gene/pseudogene); AM4 7 antibody; C C chemokine receptor type 5 antibody; C C CKR 5 antibody; C-C chemokine receptor type 5 antibody; C-C CKR-5 antibody; C-C motif chemokine receptor 5 A159A antibody; CC Chemokine Receptor 5 antibody; CC Chemokine Receptor Type 5 antibody; CC CKR 5 antibody; CC-CKR-5 antibody; CCCKR 5 antibody; CCCKR5 antibody; CCR 5 antibody; CCR-5 antibody; CCR5 antibody; CCR5 chemokine(C C motif) receptor 5 antibody; CCR5_HUMAN antibody; CD 195 antibody; CD195 antibody; CD195 Antigen antibody; Chemokine C C motif receptor 5 antibody; Chemokine receptor CCR5 antibody; CHEMR13 antibody; CKR 5 antibody; CKR5 antibody; CMKBR 5 antibody; CMKBR5 antibody; FLJ78003 antibody; HIV 1 Fusion Coreceptor antibody; HIV-1 fusion coreceptor antibody; HIV1 fusion coreceptor antibody; IDDM22 antibody; MIP-1 alpha receptor antibody

C-C chemokine receptor type 5; C-C chemokine receptor type 5; C-C chemokine receptor type 5; chemr13; HIV-1 fusion coreceptor; chemokine receptor CCR5; C-C motif chemokine receptor 5 A159A; chemokine (C-C motif) receptor 5 (gene/pseudogene); CHEMR13; HIV-1 fusion coreceptor; CD_antigen: CD195

CCR5

CCR5; CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5; CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5

CCR5_HUMAN

Polyclonal

IgG

Rabbit

Reacts with: Human. Predicted to work with: Mouse, Rat

352

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot: . Concentration: 0.1-0.5ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Immunohistochemistry (Paraffin-embedded Section): . Concentration:0.5-1ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM, pH6.0, for 20 mins is required for the staining of formalin/pafaffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CCR5 (19-34aa PCQKINVKQIAARLLP), different from the related rat sequence by two amino acids.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for C-C chemokine receptor type 5(CCR5) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: Human CC chemokine receptor 5(CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus(HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.

1705896

P51681.1

P51681

40,524 Da

Binding And Entry Of HIV Virion Pathway (105986); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Disease Pathway (530764); Early Phase Of HIV Life Cycle Pathway (105985); Endocytosis Pathway (102279)

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

CCR5: a 7-transmembrane G-linked receptor for a number of inflammatory C-C type chemokines including MIP-1-alpha, MIP-1-beta and RANTES. Transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (along with CD4) for HIV-1 R5 isolates. Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on S6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1). Variations in CCR5 gene also influence the rate of progression to AIDS after infection. R60S variant, a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5, results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors. Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection. Protein type: Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, cytokine; Membrane protein, multi-pass; GPCR, family 1. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; endosome; external side of plasma membrane. Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; coreceptor activity; C-C chemokine binding; actin binding; phosphoinositide phospholipase C activity. Biological Process: viral reproduction; MAPKKK cascade; calcium-mediated signaling; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; dendritic cell chemotaxis; cellular defense response; immune response; entry into host cell; inflammatory response. Disease: West Nile Virus, Susceptibility To; Diabetes Mellitus, Insulin-dependent, 22; Hepatitis C Virus, Susceptibility To

0.1 mg

245 USD