antibody

Anti-Alkaline Phosphatase antibody

MBS175686

0.1 mg

245 USD

polyclonal

rabbit

AP

human, mouse, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-Alkaline Phosphatase antibody

Alkaline Phosphatase

Alkaline phosphatase, tissue-nonspecific isozyme(AP-TNAP/TNSALP); alkaline phosphatase, liver/bone/kidney; Akp2 antibody; Alkaline phosphatase intestinal antibody; Alkaline phosphatase intestinal precursor antibody; Alkaline phosphatase liver/bone/kidney antibody; Alkaline phosphatase placental antibody; Alkaline phosphatase placental like 2 antibody; Alkaline phosphatase placental like antibody; Alkaline phosphatase placental type antibody; Alkaline phosphomonoesterase antibody; ALP 1 antibody; ALP antibody; ALP I antibody; ALP L antibody; ALP P antibody; ALPG antibody; ALPI antibody; ALPL antibody; ALPP antibody; ALPPL antibody; ALPPL2 antibody; AP TNAP antibody; FLJ40094 antibody; GCAP antibody; Germ cell alkaline phosphatase antibody; Glycerophosphatase antibody; HOPS antibody; IAP antibody; Intestinal alkaline phosphatase antibody; Kasahara isozyme antibody; Nagao isozyme antibody; OTTHUMP00000164357 antibody; Placental alkaline phosphatase antibody; Placental like alkaline phosphatase antibody; PLAP 1 antibody; PLAP antibody; PLAP like antibody; Regan isozyme antibody; Testicular and thymus alkaline phosphatase antibody; Tissue non specific alkaline phosphatase antibody; Tissue nonspecific ALP antibody; TNAP antibody; TNSALP antibody

Alkaline phosphatase, tissue-nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme

ALPL

ALPL; ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP; AP-TNAP; TNSALP

PPBT_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

524

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase (21-35aa,EKEKDPKYWRDQAQE), different from the related rat and mouse sequences by two amino acids.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

68067533

P05186.4

P05186

51,045 Da

AGE/RAGE Pathway (698754); BDNF Signaling Pathway (712093); Endochondral Ossification Pathway (198812); Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956); TNF-alpha/NF-kB Signaling Pathway (198884)

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.3.1; Cofactor and Vitamin Metabolism - folate biosynthesis; Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 1p36.12. Cellular Component: extracellular matrix; extracellular space; membrane; integral to membrane; plasma membrane. Molecular Function: protein binding; pyrophosphatase activity; alkaline phosphatase activity; metal ion binding. Biological Process: osteoblast differentiation; response to antibiotic; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification. Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood

0.1 mg

245 USD