Anti-GDNF antibody | MyBioSource MBS175631 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-GDNF antibody

catalog :

MBS175631

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175631

products type :

Antibody

products full name :

Anti-GDNF antibody

products short name :

GDNF

products name syn :

Glial cell line-derived neurotrophic factor(hGDNF); glial cell derived neurotrophic factor; Astrocyte derived trophic factor 1 antibody; Astrocyte derived trophic factor antibody; Astrocyte-derived trophic factor antibody; ATF 1 antibody; ATF 2 antibody; Atf antibody; ATF1 antibody; ATF2 antibody; gdnf antibody; GDNF_HUMAN antibody; Glial cell derived neurotrophic factor antibody; Glial Cell Line Derived Neurotrophic Factor antibody; Glial cell line-derived neurotrophic factor antibody; Glial derived neurotrophic factor antibody; HFB1 GDNF antibody; hGDNF antibody; HSCR3 antibody

other names :

Glial cell line-derived neurotrophic factor; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; glial cell derived neurotrophic factor; Astrocyte-derived trophic factor; ATF

products gene name :

GDNF

other gene names :

GDNF; GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF; hGDNF; ATF

uniprot entry name :

GDNF_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

211

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)

app notes :

Western Blot:. Concentration: 0.1-0.5 ug/ml. Test Species: Hu. Predicted Species: n/a. Antigen Retrieval: n/a . Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1 ug/ml. Test Species: Hu, Rat. Predicted Species: Ms. Antigen Retrieval: By Heat . Immunocytochemistry: . Concentration: 0.5-1 ug/ml. Test Species: Hu. Predicted Species: n/a. Antigen Retrieval: n/a. Test Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formal/ paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GDNF (142-158aa FRYCSGSCDAAETTYDK), different from the related mouse sequence by three amino acids and rat sequence by two amino acids.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat. Background: GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands(GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.

ncbi gi num :

729567

ncbi acc num :

P39905.1

uniprot acc num :

P39905

ncbi mol weight :

18,123 Da

ncbi pathways :

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); NCAM Signaling For Neurite Out-growth Pathway (105689); NCAM1 Interactions Pathway (105697); Signaling Events Regulated By Ret Tyrosine Kinase Pathway (137915); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

uniprot summary :

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5p13.1-p12. Cellular Component: extracellular region. Molecular Function: protein homodimerization activity; growth factor activity; receptor binding. Biological Process: positive regulation of dopamine secretion; axon guidance; nervous system development; peristalsis; adult locomotory behavior; mRNA stabilization; regulation of dopamine uptake; positive regulation of monooxygenase activity; signal transduction; enteric nervous system development; sympathetic nervous system development; ureteric bud branching; regulation of gene expression; induction of an organ; positive regulation of cell proliferation; postganglionic parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; positive regulation of cell differentiation; postsynaptic membrane organization; metanephros development; neural crest cell migration; neurite development; negative regulation of apoptosis. Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma

size1 :

0.1 mg

price1 :

245 USD

more info or order :

MyBioSource product webpage