antibody

Anti-GCLC antibody

MBS175561

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-GCLC antibody

GCLC

Glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit; EC 6.3.2.2 antibody; Gamma ECS antibody; Gamma GCS antibody; Gamma glutamylcysteine synthetase antibody; Gamma-ECS antibody; Gamma-glutamylcysteine synthetase antibody; GCL antibody; GCLC antibody; GCLM antibody; GCS antibody; GCS Heavy Chain antibody; GLCL antibody; GLCLC antibody; GLCLR antibody; Glutamate cysteine ligase catalytic subunit antibody; Glutamate cysteine ligase(gamma glutamylcysteine synthetase) regulatory(30.8kD) antibody; Glutamate cysteine ligase catalytic subunit antibody; Glutamate cysteine ligase modifier subunit antibody; Glutamate cysteine ligase regulatory protein antibody; Glutamate cysteine ligase regulatory subunit antibody; Glutamate--cysteine ligase catalytic subunit antibody; GSC light chain antibody; GSH1_HUMAN antibody

Glutamate--cysteine ligase catalytic subunit; Glutamate--cysteine ligase catalytic subunit; glutamate--cysteine ligase catalytic subunit; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase; glutamate-cysteine ligase, catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase

GCLC

GCLC; GCLC; GCL; GCS; GLCL; GLCLC; GLCL; GLCLC

GSH1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

637

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

WB:. Concentration: 0.1-0.5ug/ml. Tested Species: Rat. Concluded Species: Hu, Ms . Antigen Retrieval: n/a. IHC-P:Concentration: 0.5-1ug/ml. Tested Species: Hu, Rat. Concluded Species: Ms . Antigen Retrieval: By Heat. Other applications have not been tested. Optimal dilutions should be determined by end user.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC (6-23aa GSPLSWEETKRHADHVRR), different from the related rat and mouse sequences by one amino acid.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

1346190

P48506.2

P48506

72,766 Da

Biological Oxidations Pathway (105698); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (413393); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (468307); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Glutathione Synthesis And Recycling Pathway (105728); Keap1-Nrf2 Pathway (198777); Metabolic Pathways (132956)

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family. Protein type: Ligase; Other Amino Acids Metabolism - glutathione; EC 6.3.2.2. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: glutamate-cysteine ligase complex; cytosol. Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; coenzyme binding; ADP binding; ATP binding. Biological Process: glutamate metabolic process; response to arsenic; L-ascorbic acid metabolic process; response to hormone stimulus; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; sulfur amino acid metabolic process; response to heat; cell redox homeostasis; xenobiotic metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; response to oxidative stress; negative regulation of transcription, DNA-dependent; regulation of blood vessel size; regulation of mitochondrial depolarization; negative regulation of apoptosis. Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To

0.1 mg

245 USD