antibody

Anti-SOCS1 antibody

MBS175557

0.1 mg

315 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-SOCS1 antibody

[SOCS1]

[Suppressor of cytokine signaling 1(SOCS-1); suppressor of cytokine signaling 1; CISH 1 antibody; CISH1 antibody; Cytokine Inducible SH2 Protein 1 antibody; JAB antibody; JAK Binding Protein antibody; JAK-binding protein antibody; Janus kinase binding protein antibody; SOCS 1 antibody; SOCS-1 antibody; Socs1 antibody; SOCS1_HUMAN antibody; SSI 1 antibody; SSI-1 antibody; SSI1 antibody; STAT Induced STAT Inhibitor 1 antibody; STAT-induced STAT inhibitor 1 antibody; Suppressor of cytokine signaling 1 antibody; Supressor of cytokine Signalling 1 antibody; TEC Interacting Protein 3 antibody; Tec-interacting protein 3 antibody; TIP 3 antibody; TIP-3 antibody; TIP3 antibody]

[Suppressor of cytokine signaling 1; Suppressor of cytokine signaling 1; suppressor of cytokine signaling 1; TIP-3; JAK binding protein; JAK-binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; cytokine-inducible SH2 protein 1; suppressor of cytokine signaling 1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3]

[SOCS1]

[SOCS1; SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1; SSI1; TIP3; SOCS-1; JAB; SSI-1; TIP-3]

SOCS1_HUMAN

Polyclonal

IgG

Rabbit

Human, Rat . Predicted: Mouse

211

No cross reactivity with other proteins.

Immunogen affinity purified.

Lyophilized

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and store frozen at -20°C for longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot . Concentration: 0.1- 0.5 ug/ml. Tested Species: Human, Rat. Predicted Species: Mouse. Immunohistochemistry (Paraffin-embedded section) . Concentration: 0.5- 1 ug/ml. Tested Species: Human. Predicted Species: Mouse, Rat. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the poroduct based on sequence similarities. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin section. Other applications have not been tested. Optimal dilutions should be determined by end users.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human SOCS1, identical to the related mouse and rat sequences.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 1(SOCS1) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: Suppressor of cytokine signaling 1(SOCS1), also known as Jak-binding protein(JAB), is a negative regulator to a subset of protein-tyrosine kinases. This 211-amino acid protein has a molecular mass of about 23.5 kD. The TEC-binding region of SOCS1 resides in the N terminus. SOCS1 associates with Tec and suppresses its kinase activity. The SOCS1 gene is intronless and is mapped to on chromosome 16p13.13. SOCS1 regulates the JAK/STAT signal-transduction pathway. Moreover, it contributes to protection against hepatic injury and fibrosis, and may also protect against hepatocarcinogenesis.

20178097

O15524.1

O15524

Activated TLR4 Signalling Pathway (106400); Adaptive Immune System Pathway (366160); Adipogenesis Pathway (198832); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); ECS Complex Pathway (413455); ECS Complex Pathway (468394); EGFR1 Signaling Pathway (198782); EPO Receptor Signaling Pathway (198882)

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008]

SOCS1: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR. By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families. Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 16p13.13. Cellular Component: cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; nucleus. Molecular Function: insulin-like growth factor receptor binding; protein binding; protein kinase inhibitor activity; kinase inhibitor activity; protein kinase binding. Biological Process: fat cell differentiation; regulation of protein amino acid phosphorylation; cytokine and chemokine mediated signaling pathway; regulation of growth; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of insulin receptor signaling pathway; protein ubiquitination; negative regulation of protein kinase activity; JAK-STAT cascade; negative regulation of JAK-STAT cascade

0.1 mg

315 USD