Anti-Myeloperoxidase antibody | MyBioSource MBS175543 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Myeloperoxidase antibody

catalog :

MBS175543

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175543

products type :

Antibody

products full name :

Anti-Myeloperoxidase antibody

products short name :

Myeloperoxidase

products name syn :

Myeloperoxidase(MPO); Myeloperoxidase; 84 kDa myeloperoxidase antibody; 89 kDa myeloperoxidase antibody; EC 1.11.1.7 antibody; EC1.11.2.2 antibody; MPO antibody; Myeloperoxidase heavy chain antibody; Myeloperoxidase light chain antibody; PERM_HUMAN antibody

other names :

Myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase

products gene name :

MPO

other gene names :

MPO; MPO; MPO

uniprot entry name :

PERM_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

745

specificity :

No cross reactivity with other proteins.

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot (WB): . Concentration: 0.1-0.5 ug/ml. Tested SPecies: Hu, Rat. Predicted Species: Ms. Antigen Retrieval: n/a. Immunohistochemisty (Paraffin-embeded Section): . Concentration: 0.5-1ug/ml. Tested SPecies: Hu, Rat. Predicted Species: Ms. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Myeloperoxidase (714-728aa KNNIFMSNSYPRDFV), different from the related mouse and rat sequences by one amino acid.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Myeloperoxidase(MPO) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein though to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

ncbi gi num :

129825

ncbi acc num :

P05164.1

uniprot acc num :

P05164

ncbi mol weight :

87,248 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Folate Metabolism Pathway (198833); IL23-mediated Signaling Events Pathway (138000); Phagosome Pathway (153910); Phagosome Pathway (153859); Selenium Pathway (198825); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Vitamin B12 Metabolism Pathway (920974); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

uniprot summary :

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.11.2.2; Oxidoreductase. Chromosomal Location of Human Ortholog: 17q23.1. Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule. Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding. Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; defense response; response to yeast; response to lipopolysaccharide; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging. Disease: Myeloperoxidase Deficiency; Alzheimer Disease

size1 :

0.1 mg

price1 :

245 USD

more info or order :

MyBioSource product webpage