Anti-Apoptosis inhibitor 5 antibody | MyBioSource MBS175541 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Apoptosis inhibitor 5 antibody

catalog :

MBS175541

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-Apoptosis inhibitor 5 antibody, MBS175541, Western blotting. Lane 1: Rat Cardiac Muscle Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Testis Tissue Lysate. Lane 4: Rat Placenta Tissue Lysate. Lane 5: MCF-7 Cell Lysate. Lane 6: HELA Cell Lysate. Lane 7: CEM Cell Lysate. Lane 8: SMMC Cell Lysate. Lane 9: COLO320 Cell Lysate

image 2 :

Anti-Apoptosis inhibitor 5 antibody, MBS175541, IHC(P). IHC(P): Rat Brain Tissue

image 3 :

Anti-Apoptosis inhibitor 5 antibody, MBS175541, ICC. ICC: HELA Cell

product information

catalog number :

MBS175541

products type :

Antibody

products full name :

Anti-Apoptosis inhibitor 5 antibody

products short name :

[Apoptosis inhibitor 5]

products name syn :

[Apoptosis inhibitor 5(API-5); apoptosis inhibitor 5; AAC 11 antibody; AAC-11 antibody; AAC11 antibody; Antiapoptosis clone 11 protein antibody; API 5 antibody; API-5 antibody; API5 antibody; API5 like 1 antibody; API5_HUMAN antibody; API5L1 antibody; Apoptosis inhibitor 5 antibody; Cell migration inducing gene 8 protein antibody; Cell migration-inducing gene 8 protein antibody; Fibroblast growth factor 2 interacting factor 2 antibody; Fibroblast growth factor 2-interacting factor antibody; FIF antibody; MIG 8 antibody; MIG8 antibody; Migration inducing protein MIG8 antibody; Protein XAGL antibody]

other names :

[Apoptosis inhibitor 5; Apoptosis inhibitor 5; apoptosis inhibitor 5; FIF; antiapoptosis clone 11 protein; migration-inducing protein MIG8; cell migration-inducing gene 8 protein; fibroblast growth factor 2-interacting factor 2; apoptosis inhibitor 5; Antiapoptosis clone 11 protein; AAC-11; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL]

products gene name :

[API5]

other gene names :

[API5; API5; AAC11; AAC-11; API-5; AAC-11; FIF]

uniprot entry name :

API5_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

524

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC

app notes :

Application details contain suggested dilutions. End user should optimize the final workingconcentrations. Immunocytochemistry , 0.5-1ug/ml, Human, Mouse. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Rat, Human, Mouse. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse. Tested Species: In-house tested speceis with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions hsould be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunocytochemistry (ICC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Anti-Apoptosis inhibitor 5 antibody, MBS175541, IHC(F). IHC(F): Rat Cardiac Muscle Tissue

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5 (487-504aa KYSSNLGNFNYERSLQGK) (b), different from the related mouse sequence by one amino acid.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Apoptosis inhibitor 5(API5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat. Background: Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5(API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

ncbi gi num :

353526346

ncbi acc num :

Q9BZZ5.3

uniprot acc num :

Q9BZZ5

ncbi mol weight :

50,740 Da

ncbi summary :

This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

uniprot summary :

API5: Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Belongs to the API5 family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Apoptosis. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: nucleoplasm; spliceosome; membrane; cytoplasm; nucleus. Molecular Function: protein binding; fibroblast growth factor binding. Biological Process: apoptosis; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage