Anti-Aquaporin 3 antibody | MyBioSource MBS175443 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Aquaporin 3 antibody

catalog :

MBS175443

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human	Hattori M, Tonooka A, Zaitsu M, Mikami K, Suzue Yanagisawa A, Uekusa T, et al. Overexpression of aquaporin 1 in the tunica vaginalis may contribute to adult-onset primary hydrocele testis. Adv Urol. 2014;2014:202434 pubmed publisher

product information

catalog number :

MBS175443

products type :

Antibody

products full name :

Anti-Aquaporin 3 antibody

products short name :

Aquaporin 3

products name syn :

Aquaporin-3; aquaporin 3(Gill blood group); AQP 3 antibody; AQP-3 antibody; Aqp3 antibody; AQP3_HUMAN antibody; Aquaglyceroporin-3 antibody; Aquaporin 3(GIL blood group) antibody; Aquaporin 3(Gill blood group) antibody; Aquaporin-3 antibody; Aquaporin3 antibody; GIL antibody; Gill blood group antibody

other names :

Aquaporin-3; Aquaporin-3; aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group); aquaporin 3 (Gill blood group); Aquaglyceroporin-3

products gene name :

AQP3

other gene names :

AQP3; AQP3; GIL; AQP-3; AQP-3

uniprot entry name :

AQP3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

292

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC

app notes :

Western Blot:. Concentration: 0.1-0.5ug/ml. Tested Species: Hu, Ms, Rat. Predicted Species: n/a. Antigen Retrieval: n/a. Immunohistrochemistry (Paraffin-embedded Section):. Concentration: 0.5-1ug/ml. Tested Species: Hu,Rat. Predicted Species: Ms. Antigen Retrieval: By Heat. Immunohistrochemistry (Frozen Section): . Concentration: 0.5-1ug/ml. Tested Species: Rat. Predicted Species: Hu,Ms. Antigen Retrieval: n/a. Immunocytochemistry:. Concentration: 0.5-1ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Antigen Retrieval: n/a . Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. . Other applications have not been tested. Optimal dilutions should be determined by end users.

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3 (278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Aquaporin-3(AQP3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat. Background: AQP3, Aquaporin 3, is the third member of the major intrinsic protein family from rat kidney. AQP3 is localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7 kb. The AQP3 gene is mapped to 9p21-p12 by fluorescence in situ hybridization. AQP3 works as water-channel. And it facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree. The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms.By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.(2002) showed strong expression of an approximately 33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, 1 layer below the unstained stratum corneum(SC). AQP3 provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant water content and to prevent the formation of a continuous water gradient across the epidermis below the SC.

ncbi gi num :

2497938

ncbi acc num :

Q92482.2

uniprot acc num :

Q92482

ncbi mol weight :

29,987 Da

ncbi pathways :

Aquaporin-mediated Transport Pathway (187187); Passive Transport By Aquaporins Pathway (187188); Stabilization And Expansion Of The E-cadherin Adherens Junction Pathway (137918); Transmembrane Transport Of Small Molecules Pathway (106572); Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway (187189); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)

ncbi summary :

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]

uniprot summary :

AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Belongs to the MIP/aquaporin (TC 1.A.8) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass; Transporter, aquaporin family. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; intercellular junction. Molecular Function: transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity. Biological Process: glycerol transport; positive regulation of immune system process; cellular water homeostasis; response to retinoic acid; water transport; excretion; odontogenesis; response to vitamin D; transport; renal water homeostasis; response to calcium ion; regulation of keratinocyte differentiation; transmembrane transport. Disease: Gil Blood Group

size1 :

0.1 mg

price1 :

245 USD

more info or order :

MyBioSource product webpage