Anti-NOX2/gp91phox antibody | MyBioSource MBS175438 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-NOX2/gp91phox antibody

catalog :

MBS175438

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175438

products type :

Antibody

products full name :

Anti-NOX2/gp91phox antibody

products short name :

NOX2/gp91phox

products name syn :

Cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; AMCBX2 antibody; CGD antibody; CGD91-phox antibody; CY24B_HUMAN antibody; CYBB antibody; Cytochrome b 245, beta polypeptide antibody; Cytochrome b(558) beta chain antibody; Cytochrome b(558) subunit beta antibody; Cytochrome b-245 heavy chain antibody; Cytochrome b558 subunit beta antibody; GP91 PHOX antibody; gp91-1 antibody; gp91-phox antibody; GP91PHOX antibody; Heme-binding membrane glycoprotein gp91phox antibody; NADPH oxidase 2 antibody; Neutrophil cytochrome b 91 kDa polypeptide antibody; NOX2 antibody; p22 phagocyte B-cytochrome antibody; P91 PHOX antibody; p91-PHOX antibody; Superoxide-generating NADPH oxidase heavy chain subunit antibody

other names :

Cytochrome b-245 heavy chain; Cytochrome b-245 heavy chain; cytochrome b-245 heavy chain; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain subunit; cytochrome b-245, beta polypeptide; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome

products gene name :

CYBB

other gene names :

CYBB; CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX; NOX2; Cytochrome b558 subunit beta

uniprot entry name :

CY24B_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

570

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NOX2 (442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Cytochrome b-245 heavy chain(CYBB) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species(ROS) in a process dependent on microtubules(X-ROS signaling).

ncbi gi num :

115211

ncbi acc num :

P04839.2

uniprot acc num :

P04839

ncbi mol weight :

65,336 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing-Cross Presentation Pathway (477122); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway (477123); Disease Pathway (530764); HIF-1 Signaling Pathway (695200); Immune System Pathway (106386); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494)

ncbi summary :

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

uniprot summary :

CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections. Protein type: Membrane protein, multi-pass; EC 1.-.-.-; Membrane protein, integral; Oxidoreductase; Mitochondrial. Chromosomal Location of Human Ortholog: Xp21.1. Cellular Component: Golgi apparatus; phagocytic vesicle membrane; cell soma; mitochondrion; rough endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; NADPH oxidase complex. Molecular Function: protein binding; FAD binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; voltage-gated ion channel activity. Biological Process: response to drug; respiratory burst; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; ion transport; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; response to nutrient; hydrogen peroxide biosynthetic process. Disease: Immunodeficiency 34; Granulomatous Disease, Chronic, X-linked

size1 :

0.1 mg

price1 :

245 USD

more info or order :

MyBioSource product webpage