Anti-ALK antibody | MyBioSource MBS175323 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-ALK antibody

catalog :

MBS175323

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175323

products type :

Antibody

products full name :

Anti-ALK antibody

products short name :

ALK

products name syn :

ALK tyrosine kinase receptor; anaplastic lymphoma receptor tyrosine kinase; Alk antibody; ALK tyrosine kinase receptor antibody; ALK tyrosine kinase receptor precursor antibody; ALK/EML4 fusion gene, included antibody; ALK/NPM1 fusion gene, included antibody; ALK_HUMAN antibody; anaplastic lymphoma kinase(Ki-1) antibody; Anaplastic lymphoma kinase antibody; Anaplastic lymphoma kinase Ki 1 antibody; Anaplastic lymphoma kinase Ki1 antibody; Anaplastic lymphoma kinase p80 antibody; anaplastic lymphoma receptor tyrosine kinase antibody; CD 246 antibody; CD246 antibody; CD246 antigen antibody; EC 2.7.10.1 antibody; Ki 1 antibody; Ki1 antibody; mutant anaplastic lymphoma kinase antibody; NBLST 3 antibody; NBLST3 antibody; Tcrz antibody; TFG/ALK antibody

other names :

ALK tyrosine kinase receptor; ALK tyrosine kinase receptor; ALK tyrosine kinase receptor; CD246 antigen; mutant anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; Anaplastic lymphoma kinase; CD_antigen: CD246

products gene name :

ALK

other gene names :

ALK; ALK; CD246; NBLST3

uniprot entry name :

ALK_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1620

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ALK (1571-1589aa LFRLRHFPCGNVNYGYQQQ), identical to the related rat and mouse sequences.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for ALK tyrosine kinase receptor(ALK) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: ALK(Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246(cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.

ncbi gi num :

296439447

ncbi acc num :

Q9UM73.3

uniprot acc num :

Q9UM73

ncbi mol weight :

176,442 Da

ncbi pathways :

Non-small Cell Lung Cancer Pathway (83119); Non-small Cell Lung Cancer Pathway (531)

ncbi summary :

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

uniprot summary :

ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors. Protein type: Kinase, protein; EC 2.7.10.1; Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Alk family. Chromosomal Location of Human Ortholog: 2p23. Cellular Component: protein complex; integral to plasma membrane. Molecular Function: NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: regulation of apoptosis; cell proliferation; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; neuron development; signal transduction; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; activation of NF-kappaB transcription factor. Disease: Neuroblastoma, Susceptibility To, 3

size1 :

0.1 mg

price1 :

245 USD

more info or order :

MyBioSource product webpage