antibody

Anti-SLC10A1 antibody

MBS175267

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-SLC10A1 antibody

SLC10A1

Sodium/bile acid cotransporter; solute carrier family 10(sodium/bile acid cotransporter family), member 1; Cell growth-inhibiting gene 29 protein antibody; Growth inhibiting protein 29 antibody; Na/bile acid cotransporter antibody; Na/taurocholate transport protein antibody; Na(+)/bile acid cotransporter antibody; Na(+)/taurocholate transport protein antibody; Na/taurocholate cotransporting polypeptide antibody; NTCP antibody; NTCP_HUMAN antibody; NTCP1 antibody; SLC10A1 antibody; Sodium/bile acid cotransporter antibody; Sodium/taurocholate cotransporter antibody; Sodium/taurocholate cotransporting polypeptide antibody; Solute carrier family 10(sodium/bile acid cotransporter family) member 1 antibody; Solute carrier family 10 member 1 antibody

Sodium/bile acid cotransporter; Sodium/bile acid cotransporter; sodium/bile acid cotransporter; growth-inhibiting protein 29; Na(+)/bile acid cotransporter; sodium/taurocholate cotransporter; solute carrier family 10 member 1; Na(+)/taurocholate transport protein; cell growth-inhibiting gene 29 protein; Na/taurocholate cotransporting polypeptide; sodium/taurocholate cotransporting polypeptide; solute carrier family 10 (sodium/bile acid cotransporter family), member 1; solute carrier family 10 (sodium/bile acid cotransporter), member 1; Cell growth-inhibiting gene 29 protein; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1

SLC10A1

SLC10A1; SLC10A1; NTCP; NTCP

NTCP_HUMAN

Polyclonal

IgG

Rabbit

Human, Rat . Predict: Mouse

349

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot (WB):. Concentration: 0.1-0.5ug/ml. Tested Species: Rat. Predicted Species: Hu, Ms. Antigen Retrieval: n/a. Immunohistochemistry (IHC) Paraffin-embedded Section: . Concentration: 0.5-1ug/ml. Tested Species: Hu, Rat. Predicted Species: Ms. Antigen Retrieval: By Heat. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SLC10A1 (141-154aa YSRGIYDGDLKDKV), different from the related rat and mouse sequences by one amino acid.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Sodium/bile acid cotransporter(SLC10A1) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: SLC10A1(SOLUTE CARRIER FAMILY 10(SODIUM/BILE ACID COTRANSPORTER FAMILY), MEMBER 1),also called NTCP or NTCP1, is found in the basolateral membranes of hepatocytes, which is also involved in the reabsorption of bile acids. The SLC10A1 gene is mapped on 14q24.2. The SLC10A1 gene contains 5 exons and spans about 23 kb. Like other sodium/bile acid transporters, SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane. Injection of in vitro transcribed NTCP cRNA into Xenopus oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. NTCP-mediated taurocholate uptake in oocytes was inhibited by all major bile acid derivatives and by bromosulfophthalein.

2498046

Q14973.1

Q14973

38,119 Da

Bile Acid And Bile Salt Metabolism Pathway (106144); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Drug Induction Of Bile Acid Pathway (698755); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Recycling Of Bile Acids And Salts Pathway (106149)

The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011]

NTCP: The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium. Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter. Chromosomal Location of Human Ortholog: 14q24.1. Cellular Component: integral to plasma membrane; basolateral plasma membrane; plasma membrane. Molecular Function: bile acid:sodium symporter activity. Biological Process: bile acid and bile salt transport; bile acid metabolic process; transport; sodium ion transport; transmembrane transport

0.1 mg

245 USD