antibody

Anti-SOCS3 antibody

MBS175265

0.1 mg

315 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-SOCS3 antibody

[SOCS3]

[Suppressor of cytokine signaling 3(SOCS-3); suppressor of cytokine signaling 3; ATOD4 antibody; CIS 3 antibody; CIS-3 antibody; CIS3 antibody; Cish3 antibody; Cytokine induced SH2 protein 3 antibody; Cytokine-inducible SH2 protein 3 antibody; E2a Pbx1 target gene in fibroblasts 10 antibody; EF 10 antibody; MGC71791 antibody; SOCS 3 antibody; SOCS-3 antibody; Socs3 antibody; SOCS3_HUMAN antibody; SSI 3 antibody; SSI-3 antibody; SSI3 antibody; STAT induced STAT inhibitor 3 antibody; STAT-induced STAT inhibitor 3 antibody; Suppressor of cytokine signaling 3 antibody]

[Suppressor of cytokine signaling 3; Suppressor of cytokine signaling 3; suppressor of cytokine signaling 3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3; suppressor of cytokine signaling 3; Cytokine-inducible SH2 protein 3; CIS-3; STAT-induced STAT inhibitor 3; SSI-3]

[SOCS3]

[SOCS3; SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3; CIS3; SSI3; SOCS-3; CIS-3; SSI-3]

SOCS3_HUMAN

Polyclonal

IgG

Rabbit

Reactes with: Human. Predict to work with: Mouse

225

No cross reactivity with other proteins.

Immunogen affinity purified.

Lyophilized

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot . Concentration: 0.1- 0.5 ug/ml. Tested Species: Human. Predicted Species: Mouse . Immunohistochemistry- (Paraffin- embedded Section). Concentration: 0.5- 1 ug/ml. Tested Species: Human. Predicted Species: Mouse . Antigen Retrieval: By Heat. Tested Species: In house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SOCS3 (71-85aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 . Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 3(SOCS3) detection. Tested with WB, IHC-P in Human, Mouse. Background: SOCS3(Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor(SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon(IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

20178094

O14543.1

O14543

ATF-2 Transcription Factor Network Pathway (138006); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); ECS Complex Pathway (413455); ECS Complex Pathway (468394)

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]

SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: cytoplasm; cytosol. Molecular Function: protein binding; protein kinase inhibitor activity. Biological Process: regulation of growth; negative regulation of inflammatory response; cytokine and chemokine mediated signaling pathway; protein ubiquitination; negative regulation of protein kinase activity; negative regulation of insulin receptor signaling pathway; JAK-STAT cascade; positive regulation of cell differentiation; negative regulation of JAK-STAT cascade; negative regulation of apoptosis. Disease: Dermatitis, Atopic, 4

0.1 mg

315 USD