Polyclonal Anti-Caspase-3 (P10) | MyBioSource MBS175227 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Anti-Caspase-3 (P10)

catalog :

MBS175227

quantity :

0.1 mg

price :

250 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

catalog number :

MBS175227

products type :

Antibody

products full name :

Polyclonal Anti-Caspase-3 (P10)

products short name :

Caspase-3 (P10)

products name syn :

Caspase-3 (CASP-3); caspase 3; apoptosis-related cysteine peptidase; Apopain Cysteine protease CPP32

other names :

caspase-3 preproprotein; Caspase-3; caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1

products gene name :

CASP3

other gene names :

CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1

uniprot entry name :

CASP3_HUMAN

clonality :

Polyclonal

isotype :

Rabbit IgG

host :

Rabbit

reactivity :

Human, mouse, rat

sequence length :

277

specificity :

Human, Mouse, Rat. No cross reactivity with other proteins.

purity :

Immunogen affinity purified.

form :

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB)

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human Caspase-3 (P10)(207-220aa, RNSKDGSWFIQSLC), different from the related rat sequence by one amino acid.

other info2 :

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Rabbit IgG polyclonal antibody for Caspase-3 (CASP3) detection. Tested with WB in Human;Mouse;Rat. Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

products references :

1. Chang, J.; Wei, L.; Otani, T.; Youker, K. A.; Entman, M. L.; Schwartz, R. J. : Inhibitory cardiac transcription factor, SRF-N, is generated by caspase 3 cleavage in human heart failure and attenuated by ventricular unloading. Circulation 108: 407-413, 2003. 2. Miura, M.; Chen, X.-D.; Allen, M. R.; Bi, Y.; Gronthos, S.; Seo, B.-M.; Lakhani, S.; Flavell, R. A.; Feng, X.-H.; Robey, P. G.; Young, M.; Shi, S. : A crucial role of caspase-3 in osteogenic differentiation of bone marrow stromal stem cells. J. Clin. Invest. 114: 1704-1713, 2004. 3. Ribeil, J.-A.; Zermati, Y.; Vandekerckhove, J.; Cathelin, S.; Kersual, J.; Dussiot, M.; Coulon, S.; Moura, I. C.; Zeuner, A.; Kirkegaard-Sorensen, T.; Varet, B.; Solary, E.; Garrido, C.; Hermine, O. : Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. Nature 445: 102-105, 2007.

ncbi gi num :

14790119

ncbi acc num :

NP_004337.2

ncbi gb acc num :

NM_004346.3

uniprot acc num :

P42574

ncbi mol weight :

31,608 Da

ncbi pathways :

AGE/RAGE Pathway 698754!!Activation Of DNA Fragmentation Factor Pathway 105683!!Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway 105672!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099

ncbi summary :

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Ref.8 Ref.14. Catalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. Enzyme regulation: Inhibited by isatin sulfonamides. Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Ref.12. Subcellular location: Cytoplasm. Tissue specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Post-translational modification: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. Sequence similarities: Belongs to the peptidase C14A family.

size :

0.1 mg

price :

250 USD