antibody

Polyclonal Anti-IGF1 Receptor antibody

MBS175136

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Polyclonal Anti-IGF1 Receptor antibody

IGF1 Receptor

Insulin-like growth factor 1 receptor; insulin-like growth factor 1 receptor; CD221 antibody; CD221 antigen antibody; IGF 1 receptor antibody; IGF 1R antibody; IGF I receptor antibody; IGF-I receptor antibody; IGF1R antibody; IGF1R_HUMAN antibody; IGFIR antibody; IGFIRC antibody; IGFR antibody; Insulin like growth factor 1 receptor antibody; Insulin like growth factor 1 receptor precursor antibody; Insulin-like growth factor 1 receptor beta chain antibody; Insulin-like growth factor I receptor antibody; JTK13 antibody; MGC142170 antibody; MGC142172 antibody; MGC18216 antibody

Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor; insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD_antigen: CD221Cleaved into the following 2 chains:Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain

IGF1R

IGF1R; IGF1R; IGFR; CD221; IGFIR; JTK13; IGF-I receptor

IGF1R_HUMAN

Polyclonal

IgG

Rabbit

Reacts with: Human, Rat. Predicted to work with: Mouse

1367

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot. Concentration: 0.1-0.5 ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Immunohistochemistry (Paraffin-embedded Section). Concentration: 0.5-1ug/ml. Tested Species: Hu, Rat. Predicted Species: Ms. Antigen Retrieval: By Heat. Tested Species: In-house tested speceis with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the stianing of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor (1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Insulin-like growth factor 1 receptor(IGF1R) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: IGF1R(Insulin-like Growth Factor 1(IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1(IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al.(1998) identified a regulatory subunit of phosphatidylinositol(PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.

124240

P08069.1

P08069

154,793 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Apoptosis Pathway (198797); Endochondral Ossification Pathway (198812); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067)

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541. Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; InsR family. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: neuron projection; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; caveola; receptor complex. Molecular Function: insulin binding; identical protein binding; insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; protein-tyrosine kinase activity; insulin receptor substrate binding; phosphoinositide 3-kinase binding; G-protein alpha-subunit binding; insulin receptor binding; insulin-like growth factor receptor activity; ATP binding. Biological Process: phosphoinositide 3-kinase cascade; epidermis development; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; positive regulation of MAPKKK cascade; mammary gland development; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; axonogenesis; insulin-like growth factor receptor signaling pathway; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; negative regulation of apoptosis; positive regulation of cell migration. Disease: Insulin-like Growth Factor I, Resistance To

0.1 mg

245 USD