antibody

Anti-HSP60 antibody (monoclonal)

MBS175081

0.1 mg

315 USD

monoclonal

mouse

nonconjugated

[SJ-60]

human, rat, chicken

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-HSP60 antibody (monoclonal)

[HSP60]

[60 kDa heat shock protein, mitochondrial; heat shock 60kDa protein 1(chaperonin); SPG13; GROEL; CPN60; HuCHA60; spastic paraplegia 13(autosomal dominant); CHAPERONIN, 60-KD; Mitochondrial matrix protein P1; P60 lymphocyte protein; Heat shock protein 60; 60 kDa chaperonin; Chaperonin 60]

[60 kDa heat shock protein, mitochondrial; 60 kDa heat shock protein, mitochondrial; 60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1; heat shock 60kDa protein 1 (chaperonin); 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein]

[HSPD1]

[HSPD1; HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; HSP60; CPN60; HSP-60; Hsp60]

CH60_HUMAN

Monoclonal

IgG1

[SJ-60]

Mouse

Human, Rat, Chicken

573

No cross reactivity with other proteins

Ascites

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot: at 2-4 ug/ml with the appropriate system to detect HSP60 in cells and tissues. Immunohistochemistry (Paraffin-embedded Section): At 4-8 ug/ml to detect HSP60 in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen: Recombinant human heat shock protein 60 (HSP60)

Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Description: Mouse IgG monoclonal antibody for HSP60, heat shock 60kDa protein 1 (chaperonin) (HSPD1) detection. Tested with WB, IHC-P in Human, rat, chicken. No cross reactivity with other proteins. Uniprot ID: P10809. Background: Heat shock 60KD protein(HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.

129379

P10809.2

P10809

17,100 Da

Legionellosis Pathway (469200); Legionellosis Pathway (469186); Metabolism Of Proteins Pathway (106230); Mitochondrial Protein Import Pathway (576261); RNA Degradation Pathway (117291); RNA Degradation Pathway (116127); SIDS Susceptibility Pathways (198901); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743); Type I Diabetes Mellitus Pathway (83095)

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family. Protein type: Chaperone; Mitochondrial. Chromosomal Location of Human Ortholog: 2q33.1. Cellular Component: extracellular space; cell surface; protein complex; mitochondrion; early endosome; coated pit; cytosol; secretory granule; membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex. Molecular Function: protein binding; p53 binding; lipopolysaccharide binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; double-stranded RNA binding; unfolded protein binding; DNA replication origin binding; single-stranded DNA binding; ATP binding. Biological Process: B cell proliferation; caspase activation; B cell activation; T cell activation; protein stabilization; viral reproduction; positive regulation of apoptosis; positive regulation of interleukin-12 production; protein maturation; isotype switching to IgG isotypes; positive regulation of interleukin-6 production; B cell cytokine production; positive regulation of interleukin-10 production; response to unfolded protein; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; de novo protein folding; protein refolding; positive regulation of T cell activation; positive regulation of T cell mediated immune response to tumor cell; chaperone-mediated protein complex assembly; positive regulation of interferon-alpha production; negative regulation of apoptosis; positive regulation of macrophage activation. Disease: Spastic Paraplegia 13, Autosomal Dominant; Leukodystrophy, Hypomyelinating, 4

0.1 mg

315 USD