Anti-TSH Receptor Picoband Antibody | MyBioSource MBS1750438 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-TSH Receptor Picoband Antibody

catalog :

MBS1750438

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Figure 1. Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (MBS1750438). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.

product information

catalog number :

MBS1750438

products type :

Antibody

products full name :

Anti-TSH Receptor Picoband Antibody

products short name :

[TSH Receptor]

products name syn :

[Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R; TSHR; LGR3]

other names :

[thyrotropin receptor isoform 1; Thyrotropin receptor; thyrotropin receptor; thyroid stimulating hormone receptor; Thyroid-stimulating hormone receptor; TSH-R]

products gene name :

[TSHR]

other gene names :

[TSHR; TSHR; LGR3; CHNG1; hTSHR-I; LGR3; TSH-R]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Reacts with: Mouse, Rat. Predicted to work with: Human

sequence length :

274

specificity :

No cross reactivity with other proteins.

purity :

Immunogen affinity purified

form :

Lyophilized

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

storage stability :

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB)

app notes :

WB: 0.1-0.5ug/ml. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TSH Receptor (244-277aa KGLEHLKELIARNTWTLKKLPLSLSFLHLTRADL), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. Subcellular Localization: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in the thyroid.

other info2 :

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

products categories :

Signal Transduction; Growth Factors/Hormones; Neuroscience; endocrine system; thyroid axis

products description :

Description: TSHR (Thyroid-Stimulating Hormone Receptor), also called LGR3, is mapped to 14q31.1. The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.

ncbi gi num :

64085121

ncbi acc num :

NP_000360.2

ncbi gb acc num :

NM_000369.2

uniprot acc num :

P16473

ncbi mol weight :

86830 MW

ncbi pathways :

Arf6 Signaling Events Pathway (138034); Arf6 Trafficking Events Pathway (137954); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Hormone Ligand-binding Receptors Pathway (106369); Neuroactive Ligand-receptor Interaction Pathway (83053)

ncbi summary :

The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

uniprot summary :

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed:11847099, PubMed:12045258). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin (PubMed:12045258). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed:11847099). Plays a central role in controlling thyroid cell metabolism ().

size1 :

0.1 mg

price1 :

280 USD

more info or order :

MyBioSource product webpage