antibody

Anti-alpha-Actinin antibody (monoclonal)

MBS175023

0.1 mg

245 USD

monoclonal

mouse

nonconjugated

SA-20

human, mouse, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-alpha-Actinin antibody (monoclonal)

alpha-Actinin

Alpha-actinin-2; actinin, alpha 2; CMD1AA; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein

Alpha-actinin-2; Alpha-actinin-2; alpha-actinin-2; F-actin cross-linking protein; alpha-actinin skeletal muscle; actinin, alpha 2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein

ACTN2

ACTN2; ACTN2; CMD1AA

ACTN2_HUMAN

Monoclonal

IgG1

SA-20

Mouse

Human, Mouse, Rat, Rabbit

894

Ascites

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin

Western Blot (WB): At 3.5 ug/ml with the appropriate system to detect Alpha-actinin in cells and tissues. Immunohistochemistry (Paraffin-embedded Section): At 3.5-7ug/ml to dectect Alpha-actinin in formalin fized and paraffin embedded tissues. Boiling section is required. Immunohistochemistry (Frozen Section): at 3.5-7 ug/ml to detect Alpha-actinin in formalin or acetone fixed tissues.

Immunogen: Rabbit skeletal alpha-actinin.

Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

Description: Mouse IgG monoclonal antibody for alpha-Actinin, actinin, alpha 2 (ACTN2) detection. Tested with WB, IHC-P, IHC-F in Human, mouse, rat. No cross reactivity with other proteins. Uniprot ID: P35609. Background: Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.

543742

P35609.1

P35609

103,920 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036)

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 1q42-q43. Cellular Component: cortical actin cytoskeleton; cytoskeleton; focal adhesion; extracellular region; dendritic spine; actin filament; pseudopodium; Z disc; cytosol; filopodium. Molecular Function: actin filament binding; protein dimerization activity; integrin binding; identical protein binding; protein binding; LIM domain binding; structural constituent of muscle; cytoskeletal protein binding; titin binding; calcium ion binding; FATZ binding; thyroid hormone receptor coactivator activity. Biological Process: focal adhesion formation; platelet activation; negative regulation of potassium ion transport; positive regulation of potassium ion transport; muscle filament sliding; protein homotetramerization; regulation of apoptosis; synaptic transmission; regulation of membrane potential; platelet degranulation; microspike biogenesis; blood coagulation; cell adhesion. Disease: Cardiomyopathy, Dilated, 1aa

0.1 mg

245 USD