protein

Creatine Kinase MB (CK-MB), Human Heart

MBS173056

100 Units

250 USD

Protein

Creatine Kinase MB (CK-MB), Human Heart

Creatine Kinase MB Isoenzyme

Creatine Kinase MB (CK-MB), Human Cardiac - Lyophilized

creatine kinase M-type; Creatine kinase M-type; creatine kinase M-type; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CKCreatine kinase M-type, N-terminally processed

CKM; CKM; CKMM; M-CK; CKMM

KCRM_HUMAN

Human Heart

381

Purified

Lyophilized powder from 10 mM tris-succinate with 2 mM EDTA and 2mM DTT, pH 7.5. Appearance: Light yellow to tan powder

At -20 degree C. Storage note: 2-8 Degree C.

UNSPSC Code: 51131907. Unit Definition: One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37 degree C. Specific Activity: 35 U/mg protein. Activity: 27 U/mg (Dimension (R) Clinical Chemistry System). Solubility: Clear, tan solution (1mg/ml saline)

Reconstitution: Tris buffered saline with 1% BSA, 1mM DTT and 1mM EDTA. Recertification: October 16, 2017 . Protein: 0.78 mg protein/mg (Coomassie) . Electrophoresis: >99% CKMB (Helena QuickGel ®CK). <1% CKMM, CKBB . Contaminants: GOT: 0.04%, . LDH: 0.02% . Country of Origin: United States of America (Note: Donor material originates for TSE, CJD and vCJD-free counties, and mandatorily reports requirements for occurrence of these diseases.)

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Creatine Kinase Mb (ck-mb)

MyBioSource is the leading supplier of Human Creatine Kinase MB (CK-MB) isoenzyme for diagnostic manufacturing and research purposes. Custom preparations, technical support, bulk quantities and aliquoting available. Human Creatine Kinase is one of the most important myocardial markers (in spite of not being altogether cardiac-specific), with well-established roles in confirming acute myocardial infarction (AMI). It can also used for monitoring reperfusion during thrombolytic therapy following AMI. Testing is performed when the total Human Creatine Phosphokinase (CPK) level is elevated. Isoenzyme testing can help differentiate the source of the damaged tissue. Human CPK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type). Three different isoenzymes: Creatine Kinase MM, BB, and MB isoenzymes exist.

21536288

NP_001815.2

NM_001824.4

84,000

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Creatine Pathway (413359); Creatine Pathway (468234); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Retinoblastoma Protein Pathway (137916); Urea Cycle And Metabolism Of Amino Groups Pathway (198758)

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: cytosol. Molecular Function: creatine kinase activity; ATP binding. Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation

100 Units

250 USD

1000 Units

1010