ELISA/assay

S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit

MBS169240

96 Assays

835 USD

ELISA Kit

S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit

[S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH)]

The Kit for Human Rotavirus C (Human Rotavirus C) genomes is designed for the in vitro quantification of Human Rotavirus C genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design. The dynamics of genetic variation means that new sequence information may become available after the initial design.

Upon receipt, store the SAM Conjugate, SAH Conjugate, SAM-BSA Standard and SAH-BSA Standard at -80 degree C. Store the rest of the kit at 4 degree C.

Typical Testing Data/Standard Curve

Background/Introduction: Rotavirus is a genus of double-stranded RNA based viruses of the Reoviridae family. Species within this genus have been named A to G, all cause severe diarrhoea although species A-C are the most common in humans. The linear RNA genome of this species contains 11 segments between 613 and 3309 nucleotides in length. The three layer capsid containing this genome has icosahedral symmetry and is around 80nm in diameter and has channels which extend inward to the core. The main route of transmission is the faecal-oral route due to contamination of food and water. The virus targets the enterocytes of the intestinal villi, enters the host cell by receptormediated endocytosis and replicates in the cytoplasm. After replication and construction of the new viral progeny the cell is lysed resulting in viral release as well as release of intestinal fluid which results in diarrhoea containing viral particles. After an incubation period of around 2 days, symptoms of Rotavirus infection present with vomiting, fever and diarrhoea that can last over a week. After infection antibodies to the virus can be found in an individual, subsequent infections can occur throughout life but are mainly asymptomatic. Infection is therefore most severe in young children although newborns are thought to have acquired immunity from trans-placental transfer of antibodies. Treatment is usually directed at alleviation of symptoms and mainly involves rehydration.

96 Assays

835 USD