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OxiSelect ORAC Activity Assay Kit

MBS169125

48 Assays

310 USD

Assay Kit

OxiSelect ORAC Activity Assay Kit

[OxiSelect ORAC Activity]

[OxiSelect ORAC Activity Assay Kit]

Upon receipt store the Fluorescein Probe (100X), Antioxidant Standard, and Free Radical Initiator frozen at -20 degree C, and Assay Diluent at 4 degree C. Aliquot as necessary to avoid multiple freeze/thaws. Store all remaining kit components at room temperature until their expiration dates.

Testing Data

Testing Data #2

Samples: Urine, Serum, Plasma, Tissue Extracts, Cell Lysate, Cell Culture Media, Other Biological Fluids Samples. Detection Range: 0.5 umol/L-25 umol/L

Oxidative Stress/ Damage; Antioxidant Assays; Antioxidant Capacity Assays; ORAC (Oxygen Radical Antioxidant Capacity) Assay

Background/Introduction: Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. Thiobarbituric Acid Reactive Substances (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases TBARS Microplate Assay Kit is a sensitive assay for determining TBARS concentration in various samples. TBARS concentration is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535 nm is directly proportional to TBARS concentration in the sample.

1. Ames, B.N., Shigenaga, M.K., and Hagen, T.M. (1993) Proc. Natl. Acad. Sci. USA 90: 7915-7922. 2. Cao, G., and Prior, R. (1999) Methods Enzymol. 299: 50-62. 3. Huang, D., Ou, B., Hampsch-Woodill, M., Flanagan, J., and Prior, R. (2002) J. Agric. Food Chem. 50: 4437- 4444. 4. Huang, D., Ou, B., & Prior, R. (2005) J. Agric. Food Chem. 53: 1841-1856. 5. Ou, B., Hampsch-Woodill, M., and Prior, R. (2001) J. Agric. Food Chem. 49: 4619-4626. 6. Rice-Evans, C., and Miller, NJ. (1994) Methods Enzymol. 234: 279-293. Trolox is a trademark of Hoffman-LaRoche. 1. Yang, J. et al. (2014). Validation of Genome-Wide Association Study (GWAS)-Identified Disease Risk Alleles with Patient-Specific Stem Cell Lines. Hum Mol Genet. 23:3445-3455. 2. Ungvari, Z. et al. (2012).Testing Predictions of the Oxidative Stress Hypothesis of Aging Using a Novel Invertebrate Model of Longevity: The Giant Clam (Tridacna Derasa). J Gerontol A Biol Sci Med Sci. 10.1093/gerona/gls159. 3. Bailey-Downs, L.C. et al. (2011). Liver-Specific Knockdown of IGF-1 Decreases Vascular Oxidative Stress Resistance by Impairing the Nrf2-Dependent Antioxidant Response: A Novel Model of Vascular Aging. J. Gerontol A Biol Sci Med Sci. 10.1093/gerona/glr164. 4. Ungvari, Z. et al. (2011). Extreme Longevity Is Associated With Increased Resistance to Oxidative Stress in Arctica islandica, the Longest-Living Non-Colonial Animal. J. Gerontol A Biol Sci Med Sci. 10.1093/gerona/glr044. 5. Ungvari, Z. et al. (2011). Free Radical Production, Antioxidant Capacity, and Oxidative Stress Response Signatures in Fibroblasts from Lewis Dwarf Rats: Effects of Life Span-Extending Peripubertal GH Treatment. J. Gerontol. A Biol Sci Med Sci. 10.1093/gerona/glr004.

48 Assays

310 USD

192 Assays

560

5x192 Assays

2170