product summary
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company name :
MyBioSource
product type :
other
product name :
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Fluorometric)
catalog :
MBS168898
quantity :
100 Assays
price :
485 USD
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS168898 image 1
image 2 :
MyBioSource MBS168898 image 2
image 3 :
MyBioSource MBS168898 image 3
product information
catalog number :
MBS168898
products type :
Assay Kit
products full name :
OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Fluorometric)
products short name :
[OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate)]
products name syn :
[OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Fluorometric)]
storage stability :
Store the entire kit at -80 degree C. Avoid multiple freeze/thaws by aliquoting. The Enzyme Cofactor and 100X DAN Fluorometric Probe are light sensitive and should be maintained in amber tubes. Shipping: Ships with Dry Ice
image1 heading :
Testing Data
image2 heading :
Testing Data #2
image3 heading :
Testing Data #3
products categories :
Oxidative Stress/ Damage; Reactive Oxygen Species (ROS) Assays; In Vitro Nitric Oxide Assay
products description :
Background: Reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) work together to damage cells and have been implicated in the pathogenesis of several disease states. RNI are a family of molecules derived from nitric oxide (NO) and superoxide anion (02-), produced via nitric oxide synthase (NOS) and NADPH oxidase, respectively. Nitric oxide is an established mediator in vascular diseases, diabetes, renal ischemia, atherosclerosis, inflammatory diseases, and cancer. However, because of its extremely short halflife, direct quantitation of NO production remains challenging and unsuitable for most detection systems. Therefore, the common method for measurement of total NO has become the sum of its final oxidized products, nitrite (N02-) and nitrate (N03-) (Figure 1). OxiSelect Nitric Oxide (NitritelNitrate) Assay Kit is a simple, fluorometric assay that quantitatively measures NO in various samples by N02-IN03- determination. First, the nitrate (N03-) in the sample is converted to nitrite (N02-) by nitrate reductase enzyme (Figure 2). Next, total nitrite is detected with the fluorescent probe DAN (2,3-diaminonaphthalene). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 500 nM (in 50 !J.L sample volume).
products references :
1. Moncada, S. (1992) Acta Physiol. Scand. 145, 201-227. 2. Nathan, C. (1992) FASEB J. 6, 3051-3064. 3. Schreck, R. and Baeuerle, P.A. (1991) Trends Cell Biol. 1, 39-42. 4. Ignarro, L.J. (1990) Pharmacol. Toxicol. 67, 1-7. 5. Green, L.C., et al. (1982) Anal. Biochem. 126, 131-138.
size1 :
100 Assays
price1 :
485 USD
size2 :
5x100 Assays
price2 :
1755
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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