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product summary
company name :
MyBioSource
product type :
other
product name :
Rap2 Activation Assay Kit
catalog :
MBS168366
quantity :
5 Assays
price :
395 USD
product information
catalog number :
MBS168366
products type :
Assay Kit
products full name :
Rap2 Activation Assay Kit
products short name :
[Rap2 Activation]
products name syn :
[Rap2 Activation Assay Kit]
storage stability :
Store all kit components at -20 degree C until their expiration dates. Avoid multiple freeze/thaw cycles.
image1 heading :
Testing Data
image2 heading :
Testing Data #2
image3 heading :
Testing Data #4
products categories :
Cell Signaling and Protein Biology; G Protein Signaling / Small GTPase Assays and Vectors; Small GTPase Research Tools ; Small GTPase Activation Assays
products description :
Background: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rap, a 24 kDa protein of the Ras superfamily, regulates a variety of biological response pathways that include cell adhesion, proliferation, differentiation, and apoptosis. The Ras-like proteins Rap1 and Rap2 share 60% identity. Like other small GTPases, Rap regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In their active (GTP-bound) state, Rap1 and Rap2 bind specifically to the Rap-binding domain (RBD) of RalGDS to control downstream signaling cascades. Rap2 Activation Assay Kit utilizes RalGDS RBD Agarose beads to selectively isolate and pull-down the active form of Rap from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rap is detected by western blot analysis using an anti-Rap2 monoclonal antibody. Rap2 Activation Assay Kit provides a simple and fast tool to monitor the activation of Rap. The kit includes easily identifiable RalGDS RBD Agarose beads (see Figure 1), pink in color, and a GTPase Immunoblot Positive Control for quick Rap2 identification. Each kit provides sufficient quantities to perform 20 assays.
products references :
1. Kitayama, H., Sugimoto, Y., Matsuzaki, T., Ikawa, Y., and Noda, M. (1989) Cell 56, 77-84. 2. Matsubara, K., Kishida, S., Matsuura, Y., Kitayama, H., Noda, M., and Kikuchi, A. (1999) Oncogene 18, 1303-1312. 3. Bos, J. L., de Rooij, J., and Reedquist, K. A. (2001) Nature Rev. 2, 369-377. 4. Herrmann, C., Horn, G., Spaargaren, M., and Wittinghofer, A. (1996) J. Biol. Chem. 271, 6794-6800. Monteiro, A.C. et al. (2014). Trans-dimerization of JAM-A regulates Rap2 and is mediated by a domain that is distinct from the cis-dimerization interface. Mol Biol Cell. 25:1574-1585.
size1 :
5 Assays
price1 :
395 USD
size2 :
20 Assays
price2 :
730
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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