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OxiSelect Total Antioxidant Capacity (TAC) Assay Kit

MBS168358

20 Assays

280 USD

Assay Kit

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit

[OxiSelect Total Antioxidant Capacity (TAC)]

[OxiSelect Total Antioxidant Capacity (TAC) Assay Kit]

Upon receipt store the Reaction Buffer (100X) at 4 degree C. Store all remaining kit components at room temperature until their expiration dates.

Testing Data

Testing Data #2

Testing Data #3

Oxidative Stress/ Damage; Antioxidant Assays; Antioxidant Capacity Assays; Total Antioxidant Capacity (TAC) Assay

Background/Introduction: Oxidative stress is a physiological condition where there is an imbalance between concentrations of reactive oxygen species (ROS) and antioxidants. However, excessive ROS accumulation will lead to cellular injury, such as damage to DNA, proteins, and lipidmembranes. The cellular damage caused by ROS has been implicated in the development of many disease states, such as cancer, diabetes, cardiovascular disease, atherosclerosis, and neurodegenerative diseases. Under normal physiological conditions, cellular ROS generation is counterbalanced by the action of cellular antioxidant enzymes, macro or micro molecules, as well as other redox molecules. Antioxidants also include both hydrophilic and lipophilic molecules for metabolizing ROS. These may be localized transiently within different tissues or cells. Because of their potential harmful effects, excessive ROS must be promptly eliminated from the cells by this variety of antioxidant defense mechanisms. Although the products of ROS-induced oxidative stress are extensively used to monitor their biological effects, it is also important to evaluate the antioxidant capacity of biological fluids, cells, and extracts. Antioxidants commonly neutralize radicals via a hydrogen atom transfer (HAT) or single electron transfer (SET) mechanism. SET assays quantify the capability of an antioxidant to transfer one electron to reduce any compound, such as free radicals, carbonyls, and metals. OxiSelect Total Antioxidant Capacity (TAC) Assay Kit measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. The TAC Assay is based on the reduction of copper (II) to copper (I) by antioxidants such as uric acid. Upon reduction, the copper (I) ion further reacts with a coupling chromogenic reagent that produces a color with a maximum absorbance at 490 nm. The net absorbance values of antioxidants are compared with a known uric acid standard curve. Absorbance values are proportional to the sample's total reductive capacity. Results are expressed as "uM Copper Reducing Equivalents" or "mM Uric Acid Equivalents". Copper is advantageous over iron-based antioxidant assays because all classes of antioxidants, including thiols, are detected with marginal radical interference. In addition, the kinetics of the copper-based reaction is also faster than the iron-based reaction, which makes the TAC Assay an ideal tool for estimating reductive or antioxidant capacity efficiently and accurately. OxiSelect TAC Assay Kit is a fast and reliable kit for the direct measurement of total antioxidant capacity from cell lysate, plasma, serum, urine, tissue homogenates, and food extracts. Each kit provides sufficient reagents to perform up to 200 assays, including blanks, antioxidant standards and unknown samples. Hydrophilic and lipophilic samples are compatible with the assay. Principle of the Assay: OxiSelect TAC Assay Kit measures the total antioxidant capacity within a sample. Samples are compared to a known concentration of uric acid standard within a 96-well microtiter plate format. Samples and standards are diluted with a reaction reagent and, upon the addition of copper, the reaction proceeds for a few minutes. The reaction is stopped and read with a standard 96-well spectrophotometric microplate reader at 490 nm (Figure 1). Antioxidant capacity is determined by comparison with the uric acid standards.

1. Allard, J.P., et al. (1998) Am. J. Clin. Nutr. 67: 143-147. 2. Cerutti, P. and Trump, B. (1991) Proc. Cancer Cell 3: 1-7. 3. Frei, B., et al. (1992) "Molecular Biology of Free Radical Scavenging System" 23-45. 4. Trachootham, D., et al. (2008) Antioxid. Redox Signal. 10: 1343-1374. 5. Van-Zoeren-Grobben, et al. (1997) Acta Pediatrics. 86: 1356-1362.

20 Assays

280 USD

200 Assays

505