ELISA/assay

OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)

MBS168191

32 Assays

495 USD

ELISA Kit

OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)

[OxiSelect Oxidative DNA Damage (8-OHdG Quantitation)]

[OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation) (1 plate)]

Upon receipt, aliquot and store the 8-OHdG Standard at -20 degree C and the 8-OHdG Conjugate at -80 degree C to avoid multiple freeze/thaw cycles. Store all other components at 4 degree C until their expiration dates.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Competitive (Quantitative)

Oxidative Stress/ Damage; DNA / RNA Damage and Repair; 8-OHdG DNA Damage ELISA

Background: Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nucleic acids, proteins and lipids. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, the formation of 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the oxidative DNA damage byproducts, is physiologically formed and enhanced by chemical carcinogens. During the repair of damaged DNA in vivo by exonucleases, the resulting 8-OH-dG is excreted without further metabolism into urine. The OxiSelect™ Oxidative DNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-OHdG in urine, serum, or other cell or tissue DNA samples. The quantity of 8-OHdG in unknown sample is determined by comparing its absorbance with that of a known 8-OHdG standard curve. The kit has an 8-OHdG detection sensitivity range of 100 pg/mL to 20 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

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32 Assays

495 USD

96 Assays

925

5x96 Assays

3740