catalog number :
MBS168158
products type :
Assay Kit
products full name :
CytoSelect Leukocyte Transmigration Assay
products short name :
[CytoSelect Leukocyte Transmigration Assay]
products name syn :
[CytoSelect Leukocyte Transmigration Assay]
storage stability :
LeukoTracker Solution and TNFa should be removed from the kit and stored at -20 degree C immediately. Store all other components at 4 degree C.
image1 heading :
Testing Data
image2 heading :
Testing Data #2
image3 heading :
Testing Data #3
products categories :
Cell Based Assays; Cell Migration; Invasion and Wound Healing; Boyden Chamber Assays; Transmigration Assays
products description :
Background/Introduction: Leukocyte extravasation into perivascular tissue plays a key role in inflammatory diseases. This recruitment requires leukocyte interaction with vascular endothelium and consists of multiple, consecutive processes including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration (Figure 1). This multistep paradigm is realized by sequential activation-dependent interactions between endothelial cell adhesion molecules and their specific ligands on leukocytes. The first step of transient adhesion and rolling is known to be mediated by an interaction of leukocyte or endothelial cell selectins and their oligosaccharide-bearing ligands. Arrest and firm adhesion of leukocytes to endothelium is dependent on the activation of beta2 integrins like Mac-1 or LFA-1 on the leukocyte cell surface, followed by interaction with endothelial cell proteins belonging to the Ig superfamily such as ICAM-1. CytoSelect Leukocyte Transmigration Assay provides a robust system for the quantitative determination of leukocyte-endothelium interactions and transmigrations. The kit contains sufficient reagents for the evaluation of 24 assays in a 24-well plate.
products references :
1. Carlos T. M and Harlan J. M. (1994) Blood 84, 2068-101. 2. Meldrum, D. R. (1998) Am. J. Physiol. 274, R577-R595. 1. Zhao, L. et al. (2008). CD44 expressed on both bone marrow-derived and non-bone marrow-derived cells promotes atherogenesis in ApoE-deficient mice. Arterioscler. Thromb. Vasc. Biol. 28:1283-1289. 2. Fava, G. et al. (2008). Leptin enhances cholangiocarcinoma cell growth. Cancer Res. 68:6752-6761.