ELISA/assay

OxiSelect HNE Adduct Competitive ELISA Kit

MBS168065

32 Assays

415 USD

ELISA Kit

OxiSelect HNE Adduct Competitive ELISA Kit

[OxiSelect HNE Adduct]

[OxiSelect HNE Adduct Competitive ELISA Kit]

Upon receipt, aliquot and store the Anti-HNE Antibody, HNE-BSA Standard, HNE Conjugate and 100X Conjugate Diluent at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C.

Typical Standard Curve/Testing Data

Assay Type: Competitive

Oxidative Stress/ Damage; Lipid Peroxidation; HNE (4-Hydroxynonenal) Assays and Reagents; HNE Adduct Competitive ELISA

Principle of the Assay: First, an HNE conjugate is coated on an ELISA plate. The unknown HNE protein samples or HNEBSA standards are then added to the HNE conjugate preabsorbed ELISA plate. After a brief incubation, an anti-HNE polyclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of HNE protein adducts in unknown samples is determined by comparison with a predetermined HNE-BSA standard curve. Background/Introduction: Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural biproducts of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative stress. Both MDA and HNE have been shown to be capable of binding to proteins and forming stable adducts, also termed advanced lipid peroxidation end products. These modifications of proteins by MDA or HNE can cause both structural and functional changes of oxidized proteins. Specifically, 4-HNE can react with lysine, histidine or cysteine residues in protein to form adducts. OxiSelect HNE Adduct Competitive ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of HNE protein adducts. The quantity of HNE adduct in protein samples is determined by comparing its absorbance with that of a known HNE-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

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32 Assays

415 USD

96 Assays

770

5x96 Assays

3065