catalog number :
MBS167945
products type :
ELISA Kit
products full name :
Human Neurofilament, Light Polypeptide (NEFL) ELISA Kit
products short name :
[Neurofilament, Light Polypeptide (NEFL)]
products gene name :
[NEFL]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 5ng/L-2000ng/L. Sensitivity: 2.52ng/L
other info2 :
Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Neuro Filament Light (also known as NFL) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human NFL antibody. NFL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human NFL Antibody is added and binds to NFL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NFL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human NFL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
size4 :
10x96-Strip-Wells