catalog number :
MBS166654
products type :
ELISA Kit
products full name :
Human Sulfated-glycosaminoglycan, S-GAG ELISA Kit
products short name :
[Sulfated-glycosaminoglycan, S-GAG]
products gene name :
[S-GAG]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.2ng/ml-60ng/ml. Sensitivity: 0.15ng/ml
other info2 :
Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Sterol Regulatory Element-binding Protein 1C (also known as SREBP-1C) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SREBP-1C antibody. SREBP-1C present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SREBP-1C Antibody is added and binds to SREBP-1C in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SREBP-1C antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SREBP-1C. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
size4 :
10x96-Strip-Wells