catalog number :
MBS165883
products type :
ELISA Kit
products full name :
Rabbit platelet-derived growth factor, PDGF ELISA Kit
products short name :
[platelet-derived growth factor, PDGF]
other names :
[platelet-derived growth factor; Platelet-derived growth factor subunit B; platelet-derived growth factor subunit B; platelet derived growth factor subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; INN: Becaplermin]
products gene name :
[PDGF]
other gene names :
[PDGFB; PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; PDGF2; SIS; PDGF subunit B]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.5ng/ml - 200ng/ml. Sensitivity: 0.25ng/ml
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rabbit Platelet-derived Growth Factor (also known as PDGF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit PDGF antibody. PDGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit PDGF Antibody is added and binds to PDGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PDGFantibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit PDGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
AAA60552.1
ncbi mol weight :
25,502 Da
ncbi pathways :
Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250)
ncbi summary :
This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
uniprot summary :
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA ().
size4 :
10x96-Strip-Wells