Chicken Tumor necrosis factor alpha, TNF-alpha ELISA Kit | MyBioSource MBS165670 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken Tumor necrosis factor alpha, TNF-alpha ELISA Kit

catalog :

MBS165670

quantity :

48-Strip-Wells

price :

385 USD

product information

catalog number :

MBS165670

products type :

ELISA Kit

products full name :

Chicken Tumor necrosis factor alpha, TNF-alpha ELISA Kit

products short name :

[Tumor necrosis factor alpha, TNF-alpha]

other names :

[TNF-alpha; Tumor necrosis factor; tumor necrosis factor; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a]

products gene name :

[TNF-alpha]

other gene names :

[TNF; TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2]

reactivity :

Chicken

sequence length :

233

storage stability :

Store at 2 to 8 degree C for 6 months.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.03ng/ml-9ng/ml. Sensitivity: 0.012ng/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

products description :

Intended Uses: This sandwich kit is for the accurate quantitative detection of Chicken Tri-iodothyronine (also known as T3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Chicken T3 antibody. T3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Chicken T3 Antibody is added and binds to T3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated T3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Chicken T3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

ncbi gi num :

37210

ncbi acc num :

CAA26669.1

uniprot acc num :

P01375

ncbi mol weight :

25,644 Da

ncbi pathways :

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); AhR Pathway (755436); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509)

ncbi summary :

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

uniprot summary :

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).

size1 :

48-Strip-Wells

price1 :

385 USD

size2 :

96-Strip-Wells

price2 :

505

size3 :

5x96-Strip-Wells

price3 :

2095

size4 :

10x96-Strip-Wells

price4 :

4095